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Nikon E800 Operating Instructions
Note: These instructions were written for the microscope, equipped with a Diagnostic
Instruments SPOT camera, located in the Life Science Microscopy Facility at Purdue
University.
1. The Nikon E800 Microscope.
The Nikon E800 is a fixed-stage upright microscope configured to image in
transmitted light, DIC, and epifluorescence modes. The Nikon is equipped with the
following objectives:
2X/0.1 NA Plan Apo ------- ∞/- WD = 8.5mm
10x.0.45 NA Plan Apo DIC L ∞/0.17 WD = 4.0mm
20x/0.75 NA Plan Apo DIC M ∞/0.17 WD = 1.0mm
40x/0.9 NA Plan Apo DIC M ∞/0.17 WD = 0.11-0.23mm
100x/1.40 NA Plan Apo DIC H ∞/0.17 WD = 0.13mm Oil
The last objective (shaded) is an Oil immersion objective.
The condenser is: C-CU with 0.9 N.A. dry lens (swing-out) And contains the
following inserts: A(bright field) /DICL/ DICM/ DICH
DIC lens prisms are inserted above each individual objective lens.
The Nikon also has a filter cube slider with the following filter cubes (right to left):
1. empty (VIS)
2. Red (Rhodamine): 560±20/585/620 longpass
3. 11001 Blue (GFP longpass) 470±20/500/515 longpass
3. 41017 Green (GFP) 470±20/495/525±25 narrow band
4. 11000 Ultraviolet ( DAPI) 360±20/400/420 longpass
Camera: the microscope is equipped with a SPOT RT slider CCD camera from
Diagnostic Imaging. It can be used as a B/W camera with the color filter sets
removed or as a color camera with the filters inserted.
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