Wellcome Trust Centre for Human Genetics
Molecular Cytogenetics and Microscopy Core
Using the Nikon TE2000 Inverted Microscope
Colour image acquisition using NIS-Elements
[Basic Research] software and the Nikon
Digital Sight DS-5M Colour Camera
INSTRUCTIONS
PLEASE HANDLE CAREFULLY!
Handle the microscope gently, taking care to avoid sharp impacts.
1) TO SWITCH-ON THE MICROSCOPE AND LOOK AT YOUR SAMPLE:
Bright-field Microscopy Imaging [with the Colour Camera]:
- Turn on the transmitted light by turning on the switch on the Nikon power
supply box on the bench to the left side of the microscope.
- Press “lamp on/off” button at the base of the microscope on the left.
- Adjust the light intensity by rotating the control dial situated on the left side of
the microscope, below the on/off switch.
- For bright-field [non-fluorescence] transmission imaging: Check that the “D”
(Diffuser) and “HA” (Heat Absorber) filters at the top left of the microscope are
pushed in. Select the optional neutral density ND filters as necessary to dim the
light intensity [top right]: ND16 and ND2 – push both in for maximum light
attenuation, normally the best setting.
- The “eyepiece turret” dial should be set to O (open), when set to C it is closed.
- The "port/optical path" dial on the right side of the base should be set to port 1.
All the light now goes to the microscope eyepieces and none to the cameras.
- The "reticule in/out" lever at front of the base to the right should be turned
clockwise and set to a 2 o’clock position. This lever is under the colour camera.
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