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200 UV/VIS Series
PerkinElmer 200 UV/VIS Series User Manual
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Series 200 UV/VIS Detector
User’s Manual
2
Table of Contents
Safety and Regulatory Information
3
Information
3
Electromagnetic Compatibility (EMC
3
Europe
3
Symbols and Conventions Used in this Manual
4
Symbols Used on the Instrument
5
Warnings/Labels on the Instrument (Label Location on Rear Panel
6
Warning for Front Panel
7
Electrical Warnings - General
8
Environmental Considerations
10
Internal Warning Labels
11
Nitrogen Purge
12
Detector Flowcell
12
Storage Conditions
13
Dimensions and Weight
13
Additional Electrical Considerations and Warnings
13
Quality Control/Good Laboratory Practices
15
Definitions in Warning for Hazardous Chemicals
16
Series 200 Operating Precautions
17
Solvents
18
Solvents with Low Boiling Points
18
Buffers
18
Corrosion
19
Solvents with Auto-Ignition Temperature below 110 °C
20
Pressure Buildup
21
Warnings from the User's Manual
22
Generic Warnings
22
Moving the Detector
22
High Voltage
22
Ultraviolet Radiation
22
Table of Contents
25
Getting Started with the Detector
29
1 Series 200 UV/VIS LC Detector Overview
30
Series 200 UV/VIS LC Detector Features and Benefits
31
Notes to Remember When Using the Detector
31
Main Components of the Series 200 UV/VIS LC
32
Detector
32
Keyboard and Display
32
Input/Output Connector Panel
32
Rear Panel
34
Powering up the Detector
35
Understanding the Main Screen
36
Using the Keyboard and Display
37
Summary of the Keyboard Functions
38
Keyboard Operations
41
Instrument Specifications
48
Installation
51
2 Preparing Your Laboratory
52
Required Air Quality
52
Unpacking Your Detector
54
Items Included
54
Making Electrical Connections
55
Checking Electrical Power Requirements
55
Set
55
Ting the Operating Voltage and Installing the Fuse
55
Installing the AC Line Cord
57
Location of Electrical Connections
59
Recorder and Computer/Integrator Connections
60
Making Input/Output Connections
61
Making a Connection between Modular Cable and Twisted Pair Wires
62
Making Tubing Connections
65
Preparing the .010-Inch Tubing
65
To Make the Tubing Connections
67
Making External Tubing Connections
69
Using Manual Mode
71
3 Understanding Manual Mode
72
Viewing the Detector Manual Settings Screen
73
The Parts of the Detector Manual Settings Screen
74
Setting Operating Parameters for Immediate Change
76
Scanning the Chromatogram
78
Using Method Mode
81
4 Understanding Methods
82
Viewing the Method Screen
83
The Parts of the Method Status Screen
84
Displaying the Current Method
86
Editing the Current Method
86
Editing Timed Events
90
Creating a New Method
92
Storing a Method
93
Recalling a Method from the Directory
95
Editing a Stored Method
97
Running a Method
99
Viewing the Chromatogram
100
Using Sequences
101
5 About Sequences
102
Defining a Sequence
104
Rules for Defining a Sequence
104
Identifying the Methods to Include in a Sequence
105
Procedure for Defining a Sequence
106
Procedure for Defining a Sequence (Continued)
107
Setting up a Sequence
108
Running a Method in Sequence
109
Stopping a Sequence
110
Configuring the Detector
111
6 Setting Configuration Options
112
Setting up the Instrument
113
Diagnostic Tests for Service Use
125
Checking the Wavelength Calibration
126
Checking the Instrument Log
128
Maintenance
131
7 General Performance
132
Maintenance Guidelines
133
Preventive Maintenance
133
Routine Maintenance
134
Flowcell Maintenance
134
Photodiode and Monochromator Windows
138
Lamp Replacement
141
Troubleshooting Detector Problems
145
Gas Saturation
145
Column Contaminants
147
Troubleshooting Methods
148
Functional Test
148
Drift and Noise Problems
148
Troubleshooting Guide
151
Service
155
Principles of
157
Operation
157
8 Theory of Operation
158
Beer's Law
158
Optical Design
160
Design Considerations
162
Application Notes
163
Wavelength Selection
163
Detector Response Time
164
Enabling
167
External Control
167
9 Configuring Turbochrom with the Series 200 UV/VIS Detector
168
VIS Detector
168
Configuring the Series 200 UV/VIS Detector for External Control
168
Index
171
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PerkinElmer 200 UV/VIS Series Specifications
General
Brand
PerkinElmer
Model
200 UV/VIS Series
Category
Security Sensors
Language
English
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General
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