Millipore Millicell ERS-2 User Manual

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User Guide
Millicell
®
ERS-2
Electrical Resistance System
MERS00002
Introduction
The Millicell
®
ERS-2 Electrical Resistance System is
a meter and electrode system designed to reliably
measure Trans Epithelial Electrical Resistance (TEER)
of epithelial cells in culture. An increase in TEER
detected with the electronic circuit of the Millicell
®
ERS-2 meter and its electrode is an indication of cell
monolayer health and confluence.
A silver/silver chloride (Ag/AgCl) pellet on each
electrode tip measures voltage. The small size of
the electrode enables the user to easily measure
transepithelial voltage and the resistance of cells
grown on microporous membranes.
This system is for research use only.
Benefits of AC Power Source
Over DC Power Source
The Millicell
®
ERS-2 system uses alternating current
(AC) to make membrane resistance measurements.
Using an isolated AC source has several advantages
over the traditional use of direct current (DC):
• Membrane voltage and voltage electrode offset
do not affect resistance measurements.
• The zero net charge on the cells eliminates the
adverse effects of DC current on the cell membrane.
• There is no electrochemical deposition
of electrode metals.
• Membrane capacitance does not affect resistance
readings. Once the Millicell
®
ERS-2 system is
standardized, you can use it to quantitatively
measure cell confluence.
The Millicell
®
ERS-2 System
and Components
The Millicell
®
ERS-2 system consists of a meter,
STX01 electrode, STX04 1,000 Ω test electrode,
and battery charger.
Analog output
Digital LCD display
Mode Switch
Input Port
R Adj Calibration
Power Switch
STX01 Electrode
STX04 Test
Electrode
The Meter
The unit measures 18.4 cm × 10.8 cm × 5.8 cm
and includes an internal rechargeable 6 V NiMH
2,700 mAh battery pack with an external 12 V DC
battery charger, equipped with an AC power cord.
An analog output port allows output of analog data
to a recording device.
The Electrode
The electrode is connected to a wire with
a telephone-type plug at its end. This plug connects
to the Input port at the front of the meter during use.

Millipore Millicell ERS-2 Specifications

General

Brand	Millipore
Model	Millicell ERS-2
Category	Laboratory Equipment
Language	English