Nikon Eclipse Ti Series User Manual

Chapter
3 Principles
of DIC
Microscopy
of Dlc
M
and
Phase Contrast
,NF
ililil1
DIC microscopy
and
phase
contrast
microscopy
differ
in
terms
of their
conÎrast and
image
characleristics.
Use
lhe following
table
as a
reference in order lo
select the melhod
that
is best suited
to the specimen'
Table &1
Ditf6r6nces in the
oDtical thickness
of line
structures
determines
ths brightness
conlrast.
The
gradient
of the
optical thic*ness
determines
ths color or
brightness contrast.
Produces a
threo-dimensional
imag€.
Adjusted by
changing objectives.
(Contrast
varies according
lo thé type of
phase plala
and sp€cific absorption
of non-
diffracied light.)
Adiusred by rotaling the
polarizer.
Contrast
adiustment
Fine
obigcts are easily
detecled.
Produces
halo eflect.
Dstection s€nsitivity
has
no directionality.
Detection sensitivity
is high but has
directionality.
Contrast
is not alfected by subject size
or
phas€
datè
width.
Evsn specimens
with a
relativ€ly larga
Dhase differanca
can be obsorvsd.
No halo eltect.
lmage charactèrislics
and detection
sensitivity
Suitable
for subiects
with line struclures.
Phase difference:
DL lambda/4
or less
OM lambda/8
or less
BM
lambda or less
Best results I thickness
is nol
groater
than
10
r.
Suitable
for structures of
any
size,
whethef
fine
or
large, ewn dyed
specimens.
Accommodatgs
phase
differences of even
several
wa\relongths. Best
resulls if optical
thidc|éss
gradienl
is nol
grsalor
than lwo
ìl,avelengths.
Permits observation
even for thickn€sses
up to 0.5
mm.
(tissue
slic€s)
Suitable
sDecimens and
phase
ditference
toleraìnce
Shallonrer than
for DIC
microscopy.
2 to 3
timès ths
phase
ditferencs
(at
high
magnification)
C€nler
the annular
diaphragm and the
phase
plate
accurately.
Match lhe
dirsction in
which
lou
want to
add contrast with th6 shearing direction.
(See page
7)
Use distorlion-lre€
obiectives.
Us6
Ko€hler illumination
for b€st
results,
and make surg that
the light source
image
is enlarged adequately
on ths annular
diaphragm
plane.
Use a culture
dish,
Pelridish, €tc., wilh
glass
surlaces that
are fr€e of
irrggularities,
smooth and
parallel.
Protect thé surtacos of
cover
glasses,
slide
glassss,
condensers,
and obiaclives
from
boing
dirtied by water, oil,
lingerprints, etc. Do
not us€ l€ns-shaped
hole
glass,
etc.
Approximately as
bright as DIC
microscopy
under
white light illumination;
slightly
darksr
undsr monochromatic
illumination.
Slightly
brighter than
phase
contrasl
micro-
scopy
(under
monochromatic
illumination).
11

Nikon Eclipse Ti Series Specifications

General

Brand	Nikon
Model	Eclipse Ti Series
Category	Camera Accessories
Language	English