6
(Page)
TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION
PROCEDURE
}As the phase contrast, darkfield and simplified polarized light observations using transmitted light requires preparation using
optical devices such as an analyzer or polarizer, they will additionally be detailed in section 4, “OBSERVATION”.
Set the main switch to “ I ” (ON) and adjust the
brightness.
(Controls Used)
(P. 8)
@Main switch
²Brightness adjustment knob
Select the BF (brightfield) light path by turning
the turret.
³Turret
Select the light path (trinocular tube).
|Light path selector knob
Place the specimen on the stage.
ƒSlide holder
…X-/Y-axis knobs
Engage the 10X objective in the light path.
Then engage the top lens in the light path.
†Revolving nosepiece
‡Top lens swing-out lever
Bring the specimen in focus.
ŠCoarse/fine adjustment knobs
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.
‰Binocular tube
‹Diopter adjustment ring
ŒCondenser height adjustment
knob
™Condenser centering screws
Adjust the aperture iris and field iris diaphragms.
šAperture iris diaphragm ring
›Field iris diaphragm ring
Engage the desired objective in the light path
and bring the specimen in focus.
†Revolving nosepiece
‡Top lens swing-out lever
Insert the required filters.
œFilters
Adjust the brightness.
²Brightness adjustment knob
Start observation.
(P. 11)
(P. 12)
(P. 15)
(P. 13)
(P. 13)
(P. 15)
(P. 15)
(P. 10)
(P. 9)
(P. 15)
(P. 16)
(P. 15)
(P. 8)