5
Sample Processing - Membrane Filtration
16
Ensure that at all times you work on the fold
down
work-surface that forms an integral part
of the kit.
Keep this area as clean as possible and before
starting, wipe it down with a paper towel and a
few drops of methanol.
1 Loosen the filter funnel and remove from the
rubber base support. Invert the filter funnel and
place it down on the clean work-surface. Also
place the blue rubber base support down on
the work-surface. Ensure that these items are
only ever placed onto the clean work-surface.
2 Sterilise the forceps
by passing them
from side to side
through a flame
from a lighter and
allow to cool. Take
care not to heat
for too long as this
will cause sooty
deposits to form.
3 Remove a sterile, individually wrapped
membrane filter.
4 Peel back the transparent outer wrapper and
use the sterile forceps to separate the white,
gridded membrane filter from its blue backing
paper and remove from the outer wrapper. Only
ever grip the membrane filters at their edge.
5 Place the membrane filter directly onto the
bronze filter support disc housed in the blue
rubber base; ensure the gridded side is
face-up. If the membrane tears or becomes
contaminated, discard and use a fresh one.
• Membrane Filtration Unit
• Pistol Grip Hand Vacuum Pump
• Forceps/Tweezers
•
47mm Membrane Filters (0.45
µ
m pore size)
• MMD containing Liquid MLSB Media
• Petri Dishes containing Absorbent Pad
• Methanol (not supplied)
• Lighter/Matches (not supplied)
5.0 Membrane Filtration of the Sample
Items Required: