palintest Wagtech Potalab+ (M) User Manual

When you are in the field:
• Find a flat area! Always place kit on a
firm surface where it is easy to work
• Work in the shade where possible!
•
Use the work surface in the kits - sterilise
it before commencing and repeat as
necessary to avoid cross-contamination
• After sterilisation of the MFU, rinse the
sample cup 3 times with the sample to
remove any traces of methanol
• Remember: The MFU apparatus needs to
be sterilised BEFORE you take each new
sample - not just prior to the first sample
• Remember: After preparing samples for
incubation, label each petri dish with the
relevant information - sample no./time/
source name etc. using the pen provided
• Remember: If collecting the sample in
a bottle, store below 4ºC and analyse
within 4-6 hours
• Remember: Switch the incubator on
30 minutes before use to allow time for
incubation temperature to be reached.
The incubator will show ‘warming up’
during this initial period
• Remember: At least one hour and no more
than four hours for resuscitation periods
Ensure you have considered the following
additional items that may be required in
addition to the Potalab
®
+ (M) contents:
• Sterilised sampling bottles/Wagsac
sampling bags
• Result form/log book
• Methanol
• Non-electric autoclave/pressure cooker
• Cigarette lighter
• Paper towel
• Liquid detergent
• De-ionised water
• Waste disposal bags
• Cool box/icepacks
Before leaving for the field, ensure the
following has been carried out:
• The equipment is sterilised wherever
possible
• Sufficient petri dishes, absorbent pads,
membrane filters, culture media and
reagents are available
• Prepare MLSB media in Media Measuring
Device (MMD) - enough for one day’s
testing. If using previously prepared media
ensure the media is still bright pink and is
not cloudy
• If time is available, prepare the petri dishes
with pads before travelling to the field.
This also allows absorbent pads to avoid
contamination in the field
• If dishes are prepared before departure, an
additional one or two are recommended
for potential mishaps in the field
• Sterilise the Membrane Filtration Unit
(MFU) so it is ready for immediate use
Note: Field testing requires careful planning
8
Appendix 1 - Field Testing Check List - Hints
30

palintest Wagtech Potalab+ (M) Specifications

General

Brand	palintest
Model	Wagtech Potalab+ (M)
Category	Measuring Instruments
Language	English