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Spotlight 150
PerkinElmer Spotlight 150 User Manual
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Spotlight
150
User’
s Gui
de
MOLECULAR SPECTROSCO
PY
2
Table of Contents
Default Chapter
3
Table of Contents
3
Introduction
5
About this Manual
6
Conventions Used in this Manual
7
Notes, Cautions and Warnings
7
Definitions
10
Related Documents
10
Requirements for Using the Spotlight 150
10
Connecting the PC to the Local Area Network
10
Warnings and Safety Information
11
The Spotlight 150
12
Summary
13
General Operating Conditions
14
Electrical Safety
15
Location and Ventilation
16
Warning Labels
17
Microscope Safety Labels
17
Warning Signs on the Microscope
18
Lifting the Spotlight 150
19
EMC Compliance
20
System Requirements
21
Electrical Requirements
21
Safety Specifications
22
Microscope
22
Overview of the Spotlight 150
23
A Guided Tour of the Spotlight 150
24
Connections
25
Operation
26
The Optical System
27
Visible Light Optics
27
Infrared Optics
30
Getting Ready to Use the Spotlight 150
33
Before Using the Spotlight 150
34
Cooling the MCT Detector
35
Setting up the Spotlight 150
38
Focusing the Microscope
39
Fitting an Attenuator to the Spectrometer
41
Setting Scan Parameters
42
Preparing Samples
45
Preparing Samples
46
Tools for Sample Preparation
47
Tools Provided with the Spotlight 150
47
Other Useful Tools
48
Specialized Accessories
49
Items to Have Available
49
Common Window Materials
50
Techniques for Preparing Samples for Transmission Measurements
51
Flattening Solids
51
Slicing Samples from Solids
52
Polymers
53
Particles
55
Fibers
56
Fibrous Solids
56
Coatings on Substrates
57
Liquids
57
Techniques for Collecting Spectra
59
Techniques for Collecting Spectra
60
Using the Compression Cell
60
Collecting a Spectrum from a Thick Sample
61
Collecting a Spectrum in an Inert Atmosphere
62
Viewing a Sample with the Visible Polarizer Accessory
64
The Theory of Light Polarization
64
Applications
65
Equipment
66
Collecting IR Spectra Using the Infrared Polarizer Accessory
68
Using the Polarizer
69
Collecting Spectra with the ATR Objective
70
ATR Objective Specification
72
Fitting the ATR Crystal Holder to the Microscope
73
Measuring the Energy in Reflectance Mode
75
Adjusting the Height of the Crystal
76
Centering the Crystal
77
Checking the Infrared Alignment
78
Removing the ATR Crystal Holder
79
Fitting a Crystal Assembly to the ATR Objective
80
Manual ATR Objective
80
Automated ATR Objective
80
Cleaning the ATR Objective Crystal
82
Auto ATR Cleaning Procedure
83
Materials Needed
83
Cleaning Procedure
83
Collecting Spectra with the ATR Crystal
88
Manual ATR Objective
88
Automated ATR Objective
89
Reflectance FT-IR Microspectroscopy
91
Diffuse Reflectance
91
Specular Reflectance
92
Reflection-Absorption
93
Maintenance
94
Maintenance
95
Inspecting the Microscope
95
Protecting the Microscope
96
Cleaning
96
Cleaning the Cover
96
Replacing the Microscope Fuse
97
Service
98
Accessories
99
Sample Preparation Tools
99
Electrical Connections
100
Fitting the Plug
100
Connecting the Microscope to the Electrical Supply
101
Connecting the Microscope to the Spectrometer
101
Appendices
102
Appendix 1: Decontamination and Cleaning
103
Appendix 2: WEEE Instructions for Perkinelmer Products
104
Index
105
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PerkinElmer Spotlight 150 Specifications
General
Brand
PerkinElmer
Model
Spotlight 150
Category
Laboratory Equipment
Language
English
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