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Table of Contents

System Startup ..................................................................................................................... 3

List of devices ...................................................................................................................... 4

System Shutdown ................................................................................................................ 5

Mounting a sample ............................................................................................................... 5

Finding the sample by eye ................................................................................................... 6

Nis-Elements software layouts ............................................................................................. 7

Selecting a confocal channel setup ..................................................................................... 7

Adding a brightfield channel ................................................................................................. 8

Capture an image ................................................................................................................ 8

Pre-scan acquisition settings ............................................................................................... 9

Optimising scan settings for best image quality ................................................................. 10

Image Saturation ................................................................................................................ 12

Capture a single XY image ................................................................................................ 13

Image Window Options ...................................................................................................... 13

Adding a scale bar ............................................................................................................. 13

Capture images with Optical Zoom .................................................................................... 14

Z Stack Acquisition ............................................................................................................. 15

Z stack viewing options ...................................................................................................... 16

XYZT Experiments ............................................................................................................. 17

Multi-position XYZT Experiments ....................................................................................... 18

Tile-Scan Experiments ....................................................................................................... 19

FRAP/Bleaching Experiments ............................................................................................ 21

Appendix ........................................................................................................................... 24

Saving Images ................................................................................................................... 24

Export images in tiff format ................................................................................................ 24

Kohler Illumination ............................................................................................................. 25

Perfect Focus System (PFS) Operation ............................................................................. 26

Frequently Asked Questions .......................................................................................... 27

1. My images look noisy, what can I do to improve image quality? .................................. 27

2. I can't see anything down the microscope .................................................................... 27

3. I can't fit all of the tissue into the field of view ............................................................... 28

Nikon A1+ User Manual

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