Chapter 4 Capturing Images
12
Adjusting the exposure time
An exposure time in the range of 60 ms to 4 ms is appropriate for a normal image capturing. Adjust the light intensity
for the microscope using an ND filter to get an appropriate exposure time.
While automatic exposure is selected, the camera gain and the exposure time are automatically changed to obtain an
appropriate exposure. For this reason, a weak light intensity causes a high camera gain making the image coarse,
and a long exposure time making the focusing difficult.
ND filter
An ND filter is a filter that affects only the amount of light passed, not the color balance of the light. For
example, an “ND2” filter cuts transmitted light in half, while an “ND16” filter reduces transmitted light to 1/16th
of actual levels.
4.2.2
4.2.2 Type of F-mount Adapter and Field of View
Field of view (or angle of view) and image resolution depends on the type of F-mount adapter used to add the product to a
microscope or other optical device as shown in the table below.
Adapter type Field of view Aspect ratio Max. resolution Purpose
DS-F
F mount adapter
(direct attachment type)
A square
inscribed in a
16-mm/
22-mm/25-mm
- diameter circle
1 : 1
1608 x 1608
2136 x 2136
2424 x 2424
High sensitivity,
minimum noise imaging
DS-F2.5
F mount adapter 2.5x
A rectangle
inscribed in
a17-mm-
diameter circle
Approx.3 : 2 4908 x 3264
High resolution imaging
4.2.3
4.2.3 Calculating the Display Magnification for Microscopic Observation
The table below shows the pixel size (the maximum number of pixels stored) of image pickup device and the effective area
(area for the effective number of pixels stored) of this product.
Pixel size (m) Recorded pixel count Effective area (mm)
Width Height Width Height Width Height Diagonal
7.3 7.3 4908 3264 35.8 23.8 43.0
Use the following formula for calculating the magnification of the subject on the monitor.
Magnification on the monitor
Optical magnification (objective lens magnification relay lens magnification)
Diagonal size of monitor display
Diagonal size of effective area for image pickup device