Chapter 1 Microscopy Procedures
10
Chapter 1-2
Microscopy Procedures
Phase Contrast Microscopy
2.2
Phase Contrast Microscopy Procedure
In this procedure, only step titles are shown for operations that are the same as those of bright-field microscopy. See “2.1
Bright-field Microscopy” for details.
1. Turn on the power.
2. Lower condenser slightly from
uppermost position.
3. Fully open field and aperture
diaphragms.
4. Move the turret to the [A: empty]
position.
5. Bring the 10x Ph objective into the
optical path.
6. Bring specimen into optical path.
7. Focus on specimen.
8. Adjust diopter.
9. Adjust interpupillary.
10. Focus and center condenser.
11. Bring the Ph annular diaphragm [Ph1]
position into the optical path.
12. Center the Ph annular diaphragm.
13. Bring the desired Ph objective into the
optical path.
14. Match the Ph codes of annular
diaphragm and the objective.
15. Focus on specimen.
16. Circumscribe field diaphragm to field
of view.
17. View specimen.
18. Turn off the power.
CLAMP
TORQUE
ND4
ND8
OUT
IN
ND4
ND8
OUT
IN
PHASE CONTRAST
0.90 DRY
JA
P
AN
A
Preparation for microscopy
1
Turn on the power.
(The power LED on the front of the main body will light
up.)
2
Lower the condenser slightly from the uppermost position.
3
Fully open the field diaphragm and aperture
diaphragm.
2
5
8
9
4
10
6
12
3
13
14
1
7
15
11
16
17
18
10
3
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