Reference Section • 103
Chapter 5
We recommend that Leak Subtraction be used with caution, because it assumes that
R
seal
is constant for all voltage steps. This may not be true if, for instance, the patch
contains small channels or electrogenic transporters that do not produce discernible
single-channel events. These will appear to be part of the seal current and may impart
apparent non-linear behavior to the seal.
For subtracting leak currents in whole-cell recordings, it is safer to use a computer
program like pCLAMP, which allows off-line leak correction.
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