134 • Troubleshooting
MultiClamp 700A Theory and Operation, Copyright 2000, 2001 Axon Instruments, Inc.
device. Try setting another Device Number using the rotary selector on the back
of the MultiClamp 700A.
Symptom: Unable to adjust the Pipette Offset to zero.
Possible causes: There may be a break in the connection between the headstage
input and ground, causing the input to float. The bath may be leaking, producing
a short circuit to the microscope. In I-Clamp mode, the capacitance neutralization
circuit may be oscillating.
Suggestions: Check the electrical continuity and
DC stability of the electrode
holder and bath electrode. Check for bubbles in the microelectrode. Check that
the outside of the chamber is dry. Set Pipette Capacitance Neutralization to zero.
Symptom: Extraneous noise is present in the Scaled Output signal. Pipette Offset is
drifting rapidly.
Possible cause: The Ag/AgCl pellet or Ag wire in the electrode holder may be
defective. Dirt or corrosion may have built up in the holder or headstage
connector socket.
Suggestions: Check the
DC stability of the pellet and replace if necessary.
Rechloride the Ag wire. Clean the holder and headstage connectors.
If the problem cannot be resolved, please contact Axon Instruments for technical
support 800-635-5577 or www.moldev.com/support