II. Microscopy
34
4. Photomicrography (using a 35-mm camera mounted on the
front port)
The following describes the procedure for taking pictures with a daylight-type color film using a single-lens reflex camera
attached to the front port of the microscope. Regarding the operation of the camera, refer to the instruction manual
supplied with the camera. Load the camera with film before starting the following steps. Also refer to "14.
Photomicrography" in "III. Operation of each part."
The following instructions are based on a microscope equipped with the parts listed below.
TE2000-U microscope
T-TD eyepiece tube D
CFI 10X eyepiece
T-DH dia-illuminator 100W
LHS-H100P-1 12V100W lamphouse
12V100W halogen lamp
TE2-PS100W power supply
System condenser
LWD condenser lens
Condenser module for bright-field microscopy
Condenser module for phase-contrast microscopy
T-SR rectangular stage
T-N6 sextuple nosepiece
Objectives for bright-field microscopy
Objectives for phase-contrast microscopy
Others
1. Focus on the specimen using bright-field (BF) microscopy.
2. Set up the 35-mm camera.
1) Mount the 35-mm camera on the front port.
2) Set the “exposure mode” of the camera to < Aperture
Priority Auto Mode >.
3) Set the “exposure compensation” of the camera to < +2/3
>. (-> Slight overexposure produces better results when
using a 35-mm film camera mounted on the front port.)
4) Adjust the other settings (ISO value, photometry mode,
etc.) on the camera.
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