V. Troubleshooting
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V. Troubleshooting
If the microscope does not function properly, take appropriate action as described below. If the problem is still not
resolved after referring to “Troubleshooting,” please contact your nearest Nikon representative.
1. Optical
Symptoms Causes Countermeasures
Parts not correctly installed.
Install the parts (nosepiece, condenser, etc.)
correctly.
Movable parts not switched correctly.
Rotate the parts till the click stop position or till the
limit. (Parts such as optical path switchover dial,
nosepiece, filter sliders, condenser turret, eyepiece
tube turret and photo-mask dial.)
Stage ring on the optical path. Change the specimen position.
Field diaphragm image not focused on the
specimen surface.
Perform condenser focusing and centering correctly.
Field diaphragm stopped down too far. Open to slightly large a size as the view field.
View field
vignetting.
Uneven
brightness in the
view field.
View field not
visible.
Dirt or dust on lens and container. Clean them. Use clean container.
Dirt or dust on lens and container. Clean them. Use clean container.
Dirt or dust in the
view field.
Field diaphragm image not focused on the
specimen surface.
Perform condenser focusing and centering correctly.
Dirt or dust on lens and container. Clean them. Use clean container.
Objective’s correction ring not set to the
bottom plate thickness of the container.
Adjust correctly.
Bottom plate thickness of the container is
outside the correction range of the
objective.
Use container with bottom plate thickness within
correction range.
Poor image
quality.
Poor contrast.
Poor resolution.
Field diaphragm image not focused on the
specimen surface.
Perform condenser focusing and centering correctly.
Bright-field objective is used. Use Ph objective.
Condenser annular diaphragm not on
optical path.
Put the annular diaphragm that matches the Ph code
of the objective into the optical path.
Condenser annular diaphragm not
centered.
Center the annular diaphragm.
No phase
contrast effect.
(on Ph
microscopy)
Aperture diaphragm not fully open (when
using system condenser).
Open fully.
Uneven focus.
Revolving nosepiece not installed correctly,
or not rotated to the click stop position.
Install correctly and rotate to the click stop position.
Specimen tilted against stage surface. Correctly reposition specimen on stage.
Revolving nosepiece not installed correctly,
or not rotated to the click stop position.
Install correctly and rotate to the click stop position.
Condenser annular diaphragm not
centered.
Center the annular diaphragm.
Image flows.
Dia-illuminator tilted. Raise till it hits the limit.
No NCB11 filter in the optical path. Put it in the optical path.
Yellowish image.
Lamp voltage too low.
Adjust the brightness adjustment dial to match the
lamp ratings.
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