4
The self-test takes about 30–40 seconds. When the test is completed, the display
shows the Templates menu.
The system can be used for most applications after 15 min of lamp warm-up
but the full specifications are not obtained until after 1 hour.
Note:
4.3 Preparations before start
Buffer preparation
Prepare buffers according to ÄKTAprime plus cue cards.
Sample preparation
1
Adjust the sample composition to the binding buffer by:
•
diluting the sample in binding buffer, or
•
buffer exchange using HiTrap™ Desalting or HiPrep™ 26/10 Desalting column.
2
Filter the sample through a 0.45 µm filter.
Purification setup
Removing storage solution from the flow path
At delivery and during storage, the flow path is filled with 20% ethanol. This should be
removed before continuing the setup.
Do not use buffer with high salt concentration to flush out the ethanol. It might
cause too high backpressure.
Note:
To flush out the ethanol using deionized water:
1
Put the inlet tubing A1–A8 that is used and B in deionized water.
At delivery, only A1 and B are installed.
Note:
2
Put all waste capillaries, W1–W3, in waste.
3
Select Templates in the main menu using the and buttons and press OK.
4
Select Application Template and press OK.
5
Select System Wash Method and press OK.
34 ÄKTAprime plus Operating Instructions 28-9597-89 AB
4 Operation
4.2 Starting the instrument
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