3-
2
NOTE
Check the reagent instruction, or consult the reagent
manufacturer or distributor to see if mixing of R1 and
R2 are allowed prior to testing.
3 Click Test on the main screen to enter the test screen.
4 Select the name of the test to be run.
5 Click Water Blank, put the inlet tube into the distilled water, and click Aspirate.
6 Click Reagent Blank, put the inlet tube into the reagent blank, and click
Aspirate.
7 Select Calibrate and select calibrator in the drop down list box of S1-S8.
Dispense calibrator into the tube with reagent in it. Put the inlet tube into the
calibrator to be tested and click Aspirate. If it is multiple-point calibration, you
can proceed to the next step only when the calibration tests of all the
calibrators are completed. (If K factor is manually entered, skip it)
Put the inlet tube into the sample blank, and click Aspirate.
8 Select Sample ID and click Sample. Put the inlet tube into the container where
sample is mixed well with the reagent and click Aspirate. Repeat this to test
other samples.
9 After calibration is finished. Click QC and select controls from the drop-down
list box C1-C3. Put the inlet tube into the container where control is mixed
well with the reagent and click Aspirate.
10 After all the tests are completed, put the inlet tube into the distilled and click
Wash.
CAUTION
For flow cell, if the system switches to other tests or after test of high
concentration sample is run, wash the flow cell (for cuvette, washing
should be done after every test). Otherwise, the result might be
unreliable.
3.2.2 Cuvette Mode
The operation for cuvette mode is almost the same with that of the flow cell. The
differences are:
a) During startup initialization, you do not have to wash the tubing
b) During test, you should open the optical window and remove the cuvette ;
c) During test the reactant is dispensed into the cuvette manually. A cuvette
cap specific for cuvette should be used to cover the cuvette (see figure 3-1).
Press Aspirate button or Start to start the test.
d) After the test is completed, the cuvette should be washed manually.
e) The tubing need not to be washed during shutdown.
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