6-4
This calibration method adopts five parameters:
0
R , a , b , c and d .
This calibration method requires at least five calibrators. The concentration (or
activity) of calibrator 1 is 0, and the corresponding
is equal to
0
R .
Parabola
Calibration formula: cbCaCR ++=
2
This calibration method adopts three parameters:
a , b and c .
This calibration method requires at least three calibrators. The calibration
parameters can be calculated through the method of polynomial least squares.
Spline
Calibration formula:
32
0
)()()(
iiiiiii
CCcCCbCCaRR −+−+−+=
This calibration method requires 2 to 6 calibrators. The number of calibrators is set
to be n, so the calibration method has 4(n-1) parameters in total:
i
R
0
,
i
a ,
i
b and
i
c .
6.3 Calculating Concentration
6.3.1 Calculating Concentration of Linearly Calibrated
Sample/control
One-point linear calibration
a
R
C
=
Where,
a - calibration parameter
Two-point linear calibration
a
bR
C
−
=
Where,
a , b - calibration parameters