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Introduction...................................................................................................................................... 1

Safety Precautions (Read the following before use!)........................................................................ 1

I Name and Function of Each Part............................................................................................. 9

1 System configuration..................................................................................................... 9

2 Optical path diagram (TIRF microscopy) ..................................................................... 10

3 TIRF attachment ......................................................................................................... 11

4 TIRF cassette holder................................................................................................... 15

5 Laser unit .................................................................................................................... 16

6 Shutter control unit ...................................................................................................... 18

7 Eyepiece shutter ......................................................................................................... 20

Operations............................................................................................................................. 21

1 Getting started (Emitting the laser).............................................................................. 22

2 Operations (TIRF microscopy) .................................................................................... 24

3 Turning off power ........................................................................................................ 25

4 Simple Surface Reflection Interference Observations Using Epi-fl Attachment ............ 26

4-1 Objectives ................................................................................................................ 26

4-2 Attachment Setup Procedure................................................................................... 26

III

Maintenance.......................................................................................................................... 27

1 Replacing the halogen lamp........................................................................................ 27

2 Replacing the objective and the filter block.................................................................. 28

3 Moving or relocating the system.................................................................................. 28

4 Cleaning the lens section ............................................................................................ 28

Shutter Control Unit Communications Commands................................................................. 29

1 Communication-controlled operation ........................................................................... 29

1-1 Communications system.......................................................................................... 29

1-2 Connector specification ........................................................................................... 29

1-3 Cable specification................................................................................................... 30

1-4 Communications parameters................................................................................... 30

1-5 Communications format........................................................................................... 30

1-6 Communications sequence ..................................................................................... 31

1-7 Commands .............................................................................................................. 32

Troubleshooting..................................................................................................................... 33

Performance and Specifications ............................................................................................ 34

Nikon TIRF User Manual

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