PerkinElmer Lambda 365 User Manual

Introduction . 9
Figure 2. Energy Absorption
Once a sample absorbs light energy, the intensity of incoming light P
0
(the energy from the
light source) reduces to P by losing some of its energy;
therefore, we define the transmittance of a sample as follows:
Figure 3. Sample Absorption
As a result of absorption by a sample, transmittance is defined as;
T = P / P
0
And Absorbance is defined as:
A = -log T = log( P
0
/ P ) Absorbance is related to c (concentration) and b (pathlength of
light) according to the Lambert-Beer law and the equation;
A=εbc
Where, ε , the molar absorption coefficient of a particular molecule, varies with the
wavelength.
Although Beer's law has several limitations, it is widely applied to quantitative analysis.

PerkinElmer Lambda 365 Specifications

General

Brand	PerkinElmer
Model	Lambda 365
Category	Laboratory Equipment
Language	English