REPLIGEN XCell ATF Series User Manual

XCell
TM
ATF System with C410:V4B Controller User Guide
Contents
1.0 Description of the XCell™ ATF (Alternating Tangential Flow) System and Process .................. 1
1.1 XCell
TM
ATF System Pump Cycle .................................................................................... 3
1.2 XCell™ ATF System Control Scope and Objectives ........................................................ 5
2.0 Utility Requirements ............................................................................................................. 5
3.0 Dimensions and Weight ........................................................................................................ 6
4.0 XCell™ ATF Pump Housing Assemblies ................................................................................... 7
See Appendix 7 for list of spares and accessories ........................................................................ 12
5.0 XCell
TM
C410v4B Controller Layout ...................................................................................... 17
5.1 General Layout ............................................................................................................. 17
5.2 Filtration Assembly (Stainless Steel) ............................................................................ 18
5.3 Pneumatics Box (P-Box) Components ......................................................................... 18
5.4 Electrical Box (E-Box) ................................................................................................... 18
5.5 Power Box (Power Separation) Components .............................................................. 20
5.6 Primary Pneumatic Services......................................................................................... 20
5.7 Primary Electric Services .............................................................................................. 21
5.8 Signal ............................................................................................................................ 21
6.0 C410v4B Controller Process and Control .............................................................................. 22
6.1 Control Overview ......................................................................................................... 22
6.2 Control Functional Algorithms ..................................................................................... 24
6.3 Interface and Screens .................................................................................................. 25
6.4 Startup ......................................................................................................................... 50
6.5 Examples ...................................................................................................................... 52
7.0 XCell™ ATF Hollow Fiber Module and Diaphragm Replacement............................................ 54
7.1 Separating the Filtration Assembly from the bioreactor ............................................. 54
7.2 HFM removal................................................................................................................ 54
7.3 HFM insertion .............................................................................................................. 55
7.4 Screen Module replacement ....................................................................................... 56
7.5 Diaphragm replacement .............................................................................................. 56
7.6 Assembly ...................................................................................................................... 57
7.7 Use ............................................................................................................................... 57
8.0 Sterilization ........................................................................................................................ 58
8.1 Preparation of Filtration Assembly for Autoclaving .................................................... 58
8.2 Autoclave Cycle ............................................................................................................ 59

REPLIGEN XCell ATF Series Specifications

General

Brand	REPLIGEN
Model	XCell ATF Series
Category	Laboratory Equipment
Language	English