Conductivity Monitor - user
calibration
Follow the instruction to calibrate the conductivity flow cell constant.
ActionStep
Make sure that the instrument has been switched on for at least one hour.1
In the System Control module, on the System menu, click Settings.2
Result: The System Settings dialog box opens.
In the System Settings dialog, select Conductivity:Cond temp compensation.
Set the Compensation factor to 0, and click OK.
3
In the Calibration dialog box, in the Monitor to calibrate list, click Conduc-
tivity monitor - user calibration.
4
Prepare at least 25 ml of calibration solution. Use either a 0.1 M KCl certified
conductivity standard solution, or accurately prepare your own 1.00 M NaCl
solution.
5
Immerse a piece of sample inlet tubing, for example S1, in the calibration
solution.
6
Open the System Control module. On the Manual menu, click Execute
Manual Instructions.
7
Result: The Manual instructions dialog box opens.
In the Manual instructions dialog box:8
•
Select Flow path:Injection valve, in the Position list, click Direct inject.
Click Insert.
•
Select Flow path:Column position, in the Position list, click By-pass.
Click Insert.
•
Select Flow path:pH valve and set the Restrictor to In-line and the pH
to Off-line. Click Insert.
•
Select Flow path:Sample inlet, select a position in the Position list (in
this example S1). Click Insert.
•
Select Pumps and Pressures:Sample flow and enter 1.0 to ml/min in
the Flow rate box. Click Insert.
•
Click Execute.
Result: Calibration solution is pumped through the system by the Sample
pump.
Pump in at least 25 ml of the calibration solution, and wait until the conduc-
tivity signal is stable.
9
328 ÄKTA avant User Manual 29035184 AE
7 Maintenance
7.7 Calibration procedures
7.7.3 Calibrate the Conductivity Monitor