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AKTA avant
GE AKTA avant User Manual
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1
Intr
oduction
Purpose
of
the
User
Manual
The
User
Manual
provides
you
with
instructions
and
information
to
run
the
ÄKT
A
avant
system.
It
also
includes
r
elevant
guidance
for
practical
handling
and
maintenance
of
instrument
components.
In
this
chapter
This
chapter
contains
the
following
sections:
See
page
Section
8
1.1
Important
user
information
10
1.2
ÄK
T
A
avant
overview
12
1.3
Associated
Documentation
ÄKT
A
avant
User
Manual
29035184
AE
7
1
Introduction
6
8
Table of Contents
Default Chapter
3
Table of Contents
3
1 Introduction
7
Important User Information
8
ÄKTA Avant Overview
10
Associated Documentation
12
2 The ÄKTA Avant Instrument
15
Overview Illustrations
16
Runs in a Cold Environment
30
Removing the Foldable Door and Pump Cover
31
Liquid Flow Path
35
Instrument Display
37
Accessories
43
Column Holders
44
Tubing Holders and Other Accessories
48
Extension Box
53
3 Standard Instrument Modules
55
Pumps
56
Mixer
60
Valves, Overview
62
Inlet Valves
64
Injection Valve
70
Column Valve
73
Outlet Valve
76
Pressure Monitors
78
Ph Valve and Ph Monitor
80
3.10 Built-In Fraction Collector
83
Function
84
Illustrations of the Built-In Fraction Collector
86
Cassettes, Cassette Tray and Racks
89
3.11 UV Monitor
96
3.12 Conductivity Monitor
98
4 Optional Instrument Modules
100
System Extension Overview
101
Installation of Optional Modules
102
Extra Inlet Valves
110
Second Column Valve
114
Extra Outlet Valves
117
External Air Sensors
120
Loop Valve
124
Versatile Valve
128
Fraction Collector F9-R
130
4.10 Second Conductivity Monitor
137
4.11 Second UV Monitor
139
4.12 I/O-Box
143
Overview of the I/O-Box
144
Analog Connector and Signals
146
Digital Connector and Signals
148
Connect External Equipment to the I/O-Box
150
4.13 General System Settings
153
5 Operation
155
Before You Prepare the System
156
Prepare the Flow Path
157
Start UNICORN and Connect to the System
162
Prime Inlets and Purge Pump Heads
165
Prime Buffer Inlets and Purge System Pumps
166
Prime Sample Inlets and Purge Sample Pump
173
Prime Q Inlets
178
Connect a Column
182
Set Pressure Alarms
187
Sample Application
189
Sample Application Using Direct Injection into the Column
191
Sample Application Using a Superloop
194
Sample Application Using a Sample Loop
199
Fractionation
202
Prepare the Built-In Fraction Collector
203
Prepare Fraction Collector F9-R
210
Fractionation Overview
218
Create a Method and Perform a Run
225
Create a Method
226
Perform a Run
230
Monitor the Run
233
After Run Procedures
236
6 Performance Tests
239
General Performance Test Actions
240
Air Sensor Tests
243
Built-In Fraction Collector Test
245
Fraction Collector F9-R Test
250
Quaternary Valve Test
253
System Test
256
UV Monitor U9-L Test
260
7 Maintenance
262
Maintenance Manager
263
Maintenance Program
268
Weekly Maintenance
272
Change Pump Rinsing Solution
273
Replace the Inline Filter
275
Clean Built-In Fraction Collector Sensors
276
Monthly Maintenance
278
Check the Flow Restrictor
279
Check the Function of the Pump Flow Restrictors
281
Semiannual Maintenance
282
Clean the UV Flow Cell
283
Replace the Ph Electrode
286
Maintenance When Required
287
Clean the Instrument Externally
288
Perform System CIP
289
Perform Column CIP
297
Clean Built-In Fraction Collector
301
Clean Fraction Collector F9-R
304
Storage of the Ph Electrode
305
Clean the Ph Electrode
307
Regenerate Dried out Ph Electrode
311
Clean Pump Head Check Valves
312
Clean Pump Flow Restrictors
315
Wipe off Excess Oil from the Pump Head
317
Clean the Conductivity Flow Cell
318
Calibration Procedures
320
Calibrate the Ph Monitor
321
Calibrate the Pressure Monitors
323
Calibrate the Conductivity Monitor
327
Calibrate the UV Monitors
331
Replacement Procedures
335
Replace Tubing and Connectors
336
Replace the Mixer
338
Replace the O-Ring Inside the Mixer
340
Replace the UV Monitor U9-M Flow Cell
342
Replace UV Monitor U9-L Flow Cell
345
Replace Flow Restrictor
347
Replace the Inlet Filters
348
Replace Pump Head Check Valves
349
Replace Pump Rinsing System Tubing
352
Replace a Valve Module
354
Replace Pump Pistons, Piston Seals, O-Rings and Rinse Membranes
357
Replace Pump Pistons, Piston Seals, O-Rings and Rinse Mem- Branes
357
8 Troubleshooting
358
Introduction to Troubleshooting
359
Troubleshooting: General Checklist
361
Troubleshooting: Monitors
363
Troubleshooting: Valves
381
Troubleshooting: Fraction Collector
384
Troubleshooting: Pumps
397
Troubleshooting: Other Components
404
Troubleshooting: Instrument Communication
413
Error Codes
415
9 Reference Information
421
System Specifications
422
Module Specifications
424
Tubing and Connectors
432
Chemical Resistance Guide
440
General Information about Biocompatibility and Chemical Resistance
441
Chemical Resistance Specifications
443
Wetted Materials
447
Predefined Methods and Phases
449
System Settings
459
System Settings - UV
460
System Settings - Conductivity
461
System Settings - Ph
463
System Settings - Pumps and Pressures
464
System Settings - Air Sensor
466
System Settings - I/O-Box
467
System Settings - Fraction Collection
468
System Settings - Watch Parameters
471
System Settings - Advanced
472
System Settings - Data Collection
474
Using Manual Instructions
475
Manual Instructions - Pumps and Pressures
476
Manual Instructions - Flow Path
479
Manual Instructions - Monitors
481
Manual Instructions - Fraction Collection
483
Manual Instructions - I/O-Box
487
Manual Instructions - Alarms
488
Manual Instructions - Advanced
492
Manual Instructions - Watch Parameters
495
Manual Instructions - Other
496
Available Run Data
497
9.10 Available Curves
501
9.11 Injection Volumes and Peak Broadening
504
9.12 Delay Volumes
505
9.13 Component Volumes
509
9.14 Pressure Control
512
Check and Change the Node ID of a Module
515
10 Ordering Information
519
Index
530
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GE AKTA avant Specifications
General
Brand
GE
Model
AKTA avant
Category
Laboratory Equipment
Language
English
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