9.8.3 Manual instructions - Monitors
The table below describes the monitor related manual instructions available for ÄKTA
avant.
DescriptionInstruction name
Sets the UV signals from U9-M to 0 mAU.Auto zero UV
Sets the UV signal from U9-L, 2nd to 0 mAU.Auto zero UV 2nd
Sets the wavelengths for UV monitor U9-M. The corre-
sponding curves in the chromatogram are marked with
the set wavelengths.
Note:
•
Do not use more wavelengths than necessary for the
application.
•
At low wavelengths, the eluent can have absorbance
of its own.
Wavelength
Filters the noise in the UV signal from UV monitor U9-M.
A column-specific averaging time is set automatically
when a column is defined in a method run and Averag-
ing time is set as a variable.
Noise reduction UV
Filters the noise in the UV monitor signal from U9-L, 2nd.
A column-specific averaging time is set automatically
when a column is defined in a method run and Averag-
ing time is set as a variable.
Noise reduction UV 2nd
Facilitates monitoring using the conductivity monitor,
of a gradient, for which the user sets the conductivity
values for 0% and 100%. The Relative scale cond in-
struction can be set in ascending manner (0% for low
and 100% for high conductivity) or in descending man-
ner (0% for high and 100% for low conductivity).
Note:
The Relative scale cond instruction in descending man-
ner is especially useful for conductivity visualization in
RPC and HIC, where the conductivity curve is reversed
compared to the concentration curve (i.e., high conduc-
tivity at 0% B and low conductivity at 100% B).
Relative scale cond
ÄKTA avant User Manual 29035184 AE 481
9 Reference information
9.8 Using Manual instructions
9.8.3 Manual instructions - Monitors