Chapter 5 Troubleshooting
113
Weak contrast in phase contrast image
Problems Check Items Reference
The Ph annular diaphragm of the phase contrast slider
and the Ph code of the objective are incompatible.
Bring a Ph annular diaphragm that has the same code
as the objective's Ph code into the optical path.
2.2
3.10.1
The Ph annular diaphragm of the phase contrast slider
and the objective's phase ring image are not at
matching positions.
Adjust the position of the annular diaphragm.
2.2
3.10.1
The specimen's phase difference is too big.
Make a new specimen with a different mounting agent
or specimen thickness.
3.10.1
The specimen's phase difference and the Ph objective
type are incompatible.
Use an appropriate Ph objective for the specimen. 3.10.1
You are not using a GIF filter. Mount a GIF filter on the field lens.
2.2
3.10.1
5.1.3 Problems in Simple Polarizing Microscopy
Incomplete field of view or no field of view seen
Problems Check Items Reference
The polarizer has been mounted at a tilt. Install the polarizer so that it lies flat.
2.3
4.2.3
5.1.4 Problems in Diascopic Fluorescence Microscopy
Incomplete field of view or no field of view seen
Problems Check Items Reference
The EX filter slider is not at the click position. Move the slider until it clicks.
2.4
4.2.4
The BA filter slider is not at the click position.
Push in the slider all the way.
Move the slider until it clicks.
2.4
4.2.4
No fluorescence image seen
Problems Check Items Reference
The excitation filter is not in the optical path.
Push in the EX filter slider from the right side, bringing
the GFP position into the optical path.
2.4
4.2.4
The barrier filter is not in the optical path.
Push in the BA filter slider all the way, bringing the
barrier filter into the optical path.
2.4
4.2.4
Diascopic illumination is not bright enough.
Adjust the illumination by turning the brightness control
knob close to the maximum position.
Widen the aperture diaphragm.
3.2.1
3.8
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