Nikon ECLIPSE Si User Manual

Chapter 3 Detailed Explanation
96
3.11 Combinations of Optical Parts
Compatibility between objectives and microscopy methods
Compatibility between optical parts and microscopy methods
Objective Magnification Bright-field Phase
Contrast
Simple
Polarizing
Diascopic
Fluorescence
Dark-field
E Plan 4x 4x S - U S -
E Plan 10x 10x S - U S U
E Plan 40x 40x S - U S S
E Plan 60x 60x S - U - -
E Plan 100x Oil 100x S - U - -
Achromat DL 10x Ph1 10x S R U U U
Achromat DL 20x LWD Ph1 20x S R U U S
Achromat DL 40x Ph2 40x S R U U S
Achromat DL 100x Oil Ph3 100x S R U - -
(R: Recommended; S: Suitable; U: Usable; -: Incompatible)
20x objectives for bright-field microscopy
Plan 20x objectives can be used for bright-field microscopy with 20x magnification.
Combinations of objectives and 10x (field number 20) eyepieces
Compatibility between optical parts and microscopy methods
Objective Magnification
Total
Magnification
(x)
Numerical
Aperture
Real
Field
(mm)
Depth of
Focus
(μm)
Resolving
Power
(μm)
Working
Distance
(mm)
E Plan 4x 4x 40 0.1 5 115 2.8 30.00
E Plan 10x 10x 100 0.25 2 18.4 1.1 7.00
E Plan 40x 40x 400 0.65 0.5 2 0.4 0.65
E Plan 60x 60x 600 0.8 0.3 1.2 0.3 0.3
E Plan 100x Oil 100x 1000 1.25 0.2 0.7 0.2 0.23
Achromat DL 10x Ph1 10x 100 0.25 2 18.4 1.1 7.00
Achromat DL 20x LWD Ph1 20x 200 0.40 1 6.1 0.7 3.9
Achromat DL 40x Ph2 40x 400 0.65 0.5 2 0.4 0.65
Achromat DL 100x Oil Ph3 100x 1000 1.25 0.2 0.7 0.2 0.23

Nikon ECLIPSE Si Specifications

General

Brand	Nikon
Model	ECLIPSE Si
Category	Microscope
Language	English