Chapter 2 Microscopy Techniques
69
4 Bring the analyzer and the polarizer into the
optical path.
(1) Turn off diascopic illumination.
Press the illumination on/off button to turn off
diascopic illumination.
(2) Attach the simple polarization analyzer.
Remove the eyepiece tube, attach the simple
polarization analyzer to the dovetail for the eyepiece
tube mount, and re-attach the eyepiece tube at its
original position.
Make sure the mark showing the analyzer orientation
faces the same position as the screws on the rear of the
microscope. The (vibration) direction of the analyzer is
fixed at the microscope front and back direction.
(3) Attach the simple polarization polarizer.
Attach the simple polarization polarizer unit over the
field lens.
Make sure the mark showing the polarizer orientation
faces the front of the microscope, and keep the fixing
screw in the polarizer unit loose.
(4) Turn on diascopic illumination.
Press the illumination on/off button to turn on
diascopic illumination.
Bring the analyzer and the polarizer
into the optical path.
5 Adjust the angle of the polarizer so that the
orientations of the analyzer and polarizer are at
right angles to each other.
(1) Fully open the aperture diaphragm.
(2) Pull one eyepiece out of the eyepiece tube, and
look inside the eyepiece tube sleeve.
When you look inside the eyepiece tube sleeve, you
can see a black striped pattern showing the
polarization state. Turning the entire polarizer unit
changes the pattern.
(3) Turn the entire polarizer unit so that a dark cross
is visible inside the eyepiece tube sleeve.
If you turn the polarizer unit so that its mark faces the
front of the microscope, the polarizer is set to a
horizontal orientation, and the orientations of the
polarizer and analyzer converge at a right angle.
This state is known as “crossed Nicol.” If the
orientations successfully converge at a right angle, a
dark cross is visible inside the eyepiece tube sleeve.
(4) Tighten the fixing screw in the polarizer unit, and
fix the polarizer in place.
The fixing screw must come in contact with a
boundary protrusion of the field lens.
To fix the polarizer in an open Nicol state, remove the
fixing screw, move it to another screw hole, and
tighten the screw.
(5) Replace the centering telescope with the
eyepiece.
Turn the polarizer so that a dark cross is visible.
Open Nichol state (mark on the left side)
(To fix the polarizer in place, move the fixing screw to
another position.)
Mark
Mark
Analyze
Polarize
Mark
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