repligen.com IF.UG.028 R1
8. Operation
8.1 Running TFF/TFDF® processes
Note: Enter all TFF/TFDF® parameters on the Settings screen and select all Alarm Settings on the
Alarm Setup screen prior to starting a sequence. The system must be fully assembled, tanks
and components in place, and ready to operate.
KrosFlo® Systems are designed to run controlled TFF (Tangential Flow Filtration) and TFDF®
(Tangential Flow Depth Filtration) processes with minimal operator input. Process parameters for
Retentate flow rate and Permeate Flow are entered on the Sequences screen. The Feed Vessel
Volume and Permeate Volume setpoints are also entered on Sequences Screen. The Filter Model is
selected from the installed database on the Settings Screen.
After input of all Setup data, navigate to the Main Screen to initiate a Process run. The preferred
start-up technique is to start the TFF1 process with Pump P-01, in Manual Mode at setpoints below
parameters of the selected Auto Mode.
Once started, run the system in Manual Mode for a few minutes to completely flood the MBT and
ensure air has been displaced. Once this is complete, stop the system and run the automated
sequences from the Sequences Screen.
Once in Auto Mode, KrosFlo® Systems will maintain setpoint control during operation. Setpoints can
be changed while running during Auto operation.
The TFF/TFDF® process supports use of a Fed Batch process. Press the Fed Batch Enabled button on
the Settings screen to enable/disable the Fed Batch process.
Press STOP on the Settings or Main screens to stop the running process.
9. Generating reports
Click REPORT on the Settings screen to access the Report screen.
Figure 10. Report screen example
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