REPLIGEN XCell ATF Series User Manual (English)

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XCell

ATF System with C410:V4B Controller User Guide

106

Only one input loop shall be calibrated at a time. Each loop shall be calibrated in its entirety by

itself, without enabling (or initiating) the calibration of another loop.

The process of calibration is a 2-point linear scaling process, where a technician first confirms all

required utilities, external pressure gauge and loop wiring is connected. The following steps are

performed:

1) An enable bit is momentarily asserted. Controller is enabled in calibration mode for a

particular analog input.

2) Pneumatic devices are set to produce a minimum setting. The pressure in the system

approaches the minimum value commanded. Pressure gauge reading is allowed to

stabilize.

3) The pressure gauge reading is provided to the controller. A minimum capture bit is

momentarily asserted.

4) Pneumatic devices are set to produce a maximum setting. The value on the pressure

gauge is allowed to stabilize.

5) The pressure gauge reading is provided to the controller. A maximum capture bit is

momentarily asserted.

6) Once both minimum and maximum points are captured, the linear scaling calculation

can be performed by the PLC. This calculation is initiated by setting an accept bit

(momentarily asserted). Controller performs the linear scaling calculation and resets

the controller’s internal calibration enable bit.

Calibration Example

Technician calibrates the P2 pressure sensor.

1) Assert enable bit:

a. Set P2_Cal_Enable (input command bit) = 1 until P2_Cal_Enable (output

response bit) is set (= 1) and is received. When true…

b. Reset P2_Cal_Enable = 0.

c. Controller is enabled in calibration mode for P2 analog input.

2) Pneumatic devices are set to produce a minimum setting. (PV2=100%, PRV2=-5.00 PSI,

Solenoid=0 [vacuum])

a. While “SendingDataPage” is still equal to 3…

b. Set PV2_SP (input) = 100 Confirmed by examining DL_PV2 (output)

c. Set PRV2_SP (input) = -5 Confirmed by examining DL_PRV2 (output)

d. Reset Force_Solenoid (input) = 0 Confirmed by examining Solenoid_Forced

(output)

e. The pressure in the system approaches the minimum value commanded.

Pressure gauge reading is allowed to stabilize.

Note: P2_PV andPRV2_PV should be very near the value on the external pressure gauge.

3) Pressure gauge reading is provided to the controller.

a. Programmer provides a data entry location for technician to enter the pressure

value.

b. Set P2_P1_EU = the pressure value from the data entry location.

c. Set P2_P1_Capture = 1 until P2_P1_Captured bit is received. When true…

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REPLIGEN XCell ATF Series Specifications

General

Filtration Method	Tangential Flow Filtration (TFF)
Type	XCell ATF System
Application	Cell Culture Harvest, Clarification, Concentration, Diafiltration
Technology	Alternating Tangential Flow (ATF)
Applications	Cell Therapy, Vaccine Production
Volume Range	1 L to 2000 L
Membrane Area	0.1 m² to 10 m²
Compatibility	Compatible with various cell types and media
Dimensions	Varies by model
Weight	Varies by model
Power Requirements	100-240 VAC, 50/60 Hz