Nikon Eclipse Ni-U User Manual

Chapter 2 Troubleshooting
30
Troubleshooting
1.2
Epi-fluorescence Microscopy
Problem Cause Measure
Lack of visibility around
periphery of field of view.
Illumination is uneven
across the field of view.
Field of view is not visible.
The filter cube is misaligned.
Push the cube in to the limit.
(→See Chapter 1 “3 Assembly Method, 6 Attach the
epi-fluorescence cube turret and epi-fluorescence
attachment - ■ Attaching a filter cube” in the
“Assembly/Maintenance”.)
The shutter is closed.
Open the shutter.
(→See Chapter 3 “14.3 Protecting the Sample and
Preventing It from Decoloration (Using the Shutter)” in the
“Operation”.)
A fluorescent image is not
visible (when the lamp is
ON).
The selection of the filter cube is incorrect.
Use a correct filter cube.
(→See Chapter 3 “14.2 Selecting Filters” in the
“Operation”.)
The ND filters of the epi-fluorescence
attachment are in the optical path.
Remove the ND filters from the optical path as
necessary.
(→See Chapter 3 "14.4 Adjusting the Brightness of the
Fluorescent Image (Using ND Filters and the Aperture
Diaphragm)” in the “Operation”.)
A halogen light source is used for a dark
specimen.
Change the light source to a mercury lamp.
A designated objective is not used at UV or V
excitation.
Use a designated objective.
The fluorescent image is
very dark (when the lamp is
ON).
The room is bright. Make it darker.
The dia-illumination lamp is on. Turn off the dia-illumination lamp.
The filter cube being used is not suitable for the
specimen.
Use a filter cube suitable for the specimen.
(→See Chapter 3 “14.2 Selecting Filters” in the
“Operation”.)
The fluorescent image
quality is poor.
The objective or cover glass is dirty.
Clean it as appropriate.
(→See Chapter 3, “2.1 Cleaning Lenses” in the
“Assembly/Maintenance”.)
The immersion oil is fluorescent.
Use the non-fluorescent immersion oil.
(→See Chapter 3 “14.7 Other Considerations Concerning
Epi-Fluorescence Microscopy” in the “Operation”.)
The slide glass is fluorescent.
Use a non-fluorescent slide glass.
(→See Chapter 3 “14.7 Other Considerations Concerning
Epi-Fluorescence Microscopy” in the “Operation”.)
The contrast of the
fluorescent image is poor.
Stray light is entering from the condenser.
Lower the condenser, or remove the condenser and
attach a shielding tube.

Nikon Eclipse Ni-U Specifications

General

Brand	Nikon
Model	Eclipse Ni-U
Category	Microscope
Language	English