Chapter 2 Troubleshooting
32
Troubleshooting
Problem Cause Measure
The orientation of the polarizer is incorrect.
Adjust the orientation of the optical system correctly.
(→See Chapter 2 “4 Differential Interference Contrast
Microscopy - 14 Adjust the orientation (vibration direction)
of the polarizer and analyzer” in the “Operation”.)
A correct DIC module on the condenser has not
been selected.
Select the DIC module suitable for the objective
used.
(→See Chapter 1 “3 Assembly Method - 13 Attach the
condenser, ■ Attaching the optical module to the universal
condenser”, and “■Attaching the DIC module to DIC
condenser oil” in the “Assembly/Maintenance”.)
Incorrect combination of the objective and the
DIC slider on the objective.
Use the DIC slider suitable for the objective.
(→See Chapter 1 “3 Assembly Method - 13 Attach the
condenser, ■ Attaching the optical module to the universal
condenser”, and “■Attaching the DIC module to DIC
condenser oil” in the “Assembly/Maintenance”.)
Dusty objective, condenser, and specimen.
Gently wipe dust off. (Special attention should be
paid to dust.)
(→See Chapter 3, “2.1 Cleaning Lenses” in the
“Assembly/Maintenance”.)
The field diaphragm image is not focused on the
specimen surface.
Make sure the condenser is focused and centered.
(→See Chapter 3 “5 Focusing and Centering the
Condenser” in the “Operation”.)
Poor contrast.
The immersion oil is not sufficient in the oil
condenser, and air bubbles are between the
slide and the condenser.
Apply the immersion oil again.
(→See Chapter 3 “12 Oil Immersion/Water Immersion” in
the “Operation”.)
1.4
Phase Contrast Microscopy
Problem Cause Measure
The PH module of the condenser does not
match the phase plate image of the objective.
Adjust so that they match.
(→See Chapter 2 “5 Phase Contrast Microscopy - 14
Center the PH module” in the “Operation”）
The PH module of the condenser does not
match the Ph code of the objective.
Put the PH module with the same Ph code as the
objective into the optical path.
(→See Chapter 2 “5 Phase Contrast Microscopy - 16
Adjust the PH module in the condenser with the Ph
objective to be used” in the “Operation”.)
The phase contrast of the specimen is too large.
Change the mounting agent or thickness of the
specimen when preparing the specimen.
(→See Chapter 3 “16.1 Tips for Phase Contrast
Microscopy” in the “Operation”.)
Poor contrast.
The type of the Ph objective is not suitable for
phase contrast of the specimen.
Use a Ph objective suitable for the specimen.
(→See Chapter 3 “16.1 Tips for Phase Contrast
Microscopy” in the “Operation”.)
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