7.3 System test failure
Description
The System test checks the function of the liquid delivery and the UV and conductivity
monitors. Correct gradient formation is tested by producing a linear gradient and a series
of concentration steps of acetone. Correct UV monitoring is tested by monitoring the
absorbance ratios 265/254 nm and 265/280 nm with 1% acetone concentration.
The outcome of each component of the test is listed individually in the performance test
report.
Corrective actions
Corrective actions in the case of failure are listed in the table below. If the problem persists
after corrective action has been applied, contact your GE representative.
It is important that the correct concentrations (1% (v/v) acetone and 1 M NaCl)
are used in the System test and that the solutions are thoroughly mixed.
Note:
Corrective actionFailed test
Make sure that tubing to and from Pump A and Pump B
is not obstructed.
Gradient test
Restart the instrument to perform automatic wavelength
calibration and light intensity check for the UV monitor.
Make sure that tubing to and from Pump A and Pump B
is not obstructed.
Step response test
Errors in all values may indicate air in the pump. Errors
in the 5% or 95% values indicate a problem with Pump B
or Pump A respectively. Run the Pressure pulsation test
to test the pump status.
Restart the instrument to perform automatic wavelength
calibration and light intensity check for the UV monitor.
Clean the UV flow cell.UV response test
Restart the instrument to perform automatic wavelength
calibration and light intensity check for the UV monitor.
Restart the instrument to perform automatic wavelength
calibration and light intensity check for the UV monitor.
Curve amplitude stability
(UV)
ÄKTA pilot 600 User Manual 29274559 AA 131
7 Troubleshooting
7.3 System test failure
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