4.1.2 Apply sample from a system inlet
Introduction
Samples may be applied from a system inlet using the system pump A. Up to three Inlet
valve A modules are supported, giving up to nine inlets, labeled A1 to A9. Inlet A1 is
normally used for buffer: other inlets may be used for sample or different buffers as re-
quired.
Sample application from B inlets is not recommended.
Note:
There are up to three stages in sample application from a sample inlet.
DescriptionStage
Prime the inlet tubing with sample. This will fill the inlet tubing up to the inlet
valve with sample, giving more precise control over the amount of sample
applied to the column.
1
The inlet tubing may be primed automatically (see Sample Application phase
properties, on page 63) or manually (see Priming the inlet tubing manually,
on page 66).
Apply the sample according to the phase properties in the Sample Applica-
tion phase.
2
Finalize the sample application (if selected in the Sample Application phase).
This will chase the sample remaining in the flow path on to the column, using
the buffer inlet as specified in Method Settings.
3
When to use
Sample application from a system inlet is recommended for sample volumes larger than
50 mL.
Sample Application phase
properties
To apply sample from a system inlet, select Inject sample directly onto column in the
Phase Properties for the Sample Application phase. Set the parameters as described
in the table below. For details of settings in the Phase Properties that are not described
here, see the UNICORN online help.
ÄKTA pilot 600 User Manual 29274559 AA 63
4 Operation
4.1 Sample application
4.1.2 Apply sample from a system inlet
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