4.4 Dual pump flow
Introduction
It is possible to use both pumps to obtain a flow rate in the range 600 to 1200 mL/min.
This function is called Dual pump flow.
Isocratic applications
When Dual pump flow is used during isocratic applications, the flow rate can be set to
up to 1200 mL/min.
In isocratic applications the same buffer is used for pump A and B. This can
be achieved by placing the A and B inlets in the same buffer container.
Note:
Gradient applications
It is possible to use Dual pump flow to make gradients above 600 mL/min. The illustration
below shows the gradient range for ÄKTA pilot 600. Above 600 mL/min (yellow part of
the curve), the gradient range is limited due to each pump maximum flow rate of
600 mL/min. For example, at 720 mL/min (dashed line in the illustration) a gradient can
be run between 20% and 80% B.
0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
100%
0 200 400 600 800 1000 1200
% B
mL/min
Gradient applications require that the inlets for pumps A and B are placed in
different buffers.
Note:
ÄKTA pilot 600 User Manual 29274559 AA 81
4 Operation
4.4 Dual pump flow
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