ActionStep
When the tubing from the buffer tanks is connected to the corresponding
inlets, carefully open the clamp at the system inlet valve connection to
prime/fill the tubing as much as possible. When the liquid has reached the
connection, tighten the clamp again.
Note:
This procedure is performed to avoid pumping too much air into the system.
4
When applicable, check that the air sensor is connected at the inlet that will
be used as the sample inlet.
5
When applicable, insert filter cartridge(s) into the in-line filter housing.6
When applicable, insert the pH electrode (packed separately) into the pH
flow cell as described in Install the pH electrode, on page 115.
7
Run priming and leakage test
The priming and leakage test steps are performed by using the Manual control module
in the UNICORN process control software, as described below.
ActionStep
Open an appropriate inlet and outlet, e.g., Valves:InletA1 and Valves:Outlet1.1
If a column is connected to the system, check that the column is bypassed
(e.g., Valves:Column:Bypass_Both).
2
Using the pressure sensor located closest to the inlet used, check that the
pressure alarm is set less than or equal to the maximum allowed system
pressure.
3
Furthermore, the air sensor alarms before the column must be disabled
(Alarms:Air_Alarm:Disabled).
Start the pump (without feedback) at approximately 20% of maximum ca-
pacity (Pump:ManFlow:20%).
4
Check that the air trap is in-line (Valves:AirTrap:Inline) and check that auto-
matic level control is enabled Alarms:AirTrapLevelControl:Enabled).
5
When applicable, put the filter in-line (Valves:Filter:Inline) and then open
the manual valve on top of the filter housing. When liquid comes out through
the manual valve, close the manual valve.
6
Increase the flow rate to 50% and flush all flow paths and inlets/outlets
thoroughly.
7
ÄKTAprocess Operating Instructions 29-0252-49 AA 119
5 Operation
5.1 Prepare the system
5.1.3 Priming and leakage test
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