5.1.3 Priming and leakage test
Introduction
Before a run is started the system should be primed and tested for possible leakage.
This section describes a procedure for priming and testing for leakage.
Important
NOTICE
All system parts must be correctly filled with liquid before any
functional tests are performed. A suitable liquid is a storage or
running buffer for the column.
NOTICE
During the procedure described below, check all valves and tubing
connections carefully for air or liquid leakage. If there is any evi-
dence of leakage, attend to the leak and then repeat the filling se-
quence.
Each pump must be tested separately if more than one is used (Optional Gra-
dient pump B and/or Sample pump in addition to the standard pump A).
Note:
Prepare for priming and leakage
test
Follow the instruction below to prepare ÄKTAprocess for priming and leakage test.
ActionStep
Check that the inlet and outlet tubing are correctly connected.1
Check that the buffer tank(s) are filled with a sufficient amount of a suitable
liquid for the procedure.
2
Check that valves to the buffer tanks are open.3
118 ÄKTAprocess Operating Instructions 29-0252-49 AA
5 Operation
5.1 Prepare the system
5.1.3 Priming and leakage test
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