GE Analytical Instruments ©2006 10-2 DLM 14291 Rev. A
• A Luer tee for connection of the NOA’s gas sampling line.
• A nasal olive equipped with tubing to connect to the Luer tee.
• A second nasal olive connected to a supply of low NO air.
• The Accurate NO Breath Kit or another device such as a balloon for elevating
the soft palette.
Suitable vacuum sources include house vacuum or vacuum pump capable of
drawing gas at the required flow rates. Flow into the pump must be controlled
using some type of flow control device. Rotometers are available from a number of
sources (e.g. Aalborg Instruments & Controls, Inc., Alltech Associates, Inc.,
Supelco Inc.) and provide for both flow control and measurement. Needle valves
and other flow controllers can be used, but require some flow measurement
system for adjusting the flow.
The nasal olives should be composed of a soft, non-traumatizing material. Soft
rubber one-hole stoppers have also been used in place of nasal olives.
Performing the Maneuver
• Connect the vacuum line to one leg of the Luer tee, connect the NOA’s gas
sampling line to another leg and the line to the nasal olive the final leg of the
tee.
• Adjust the flow rate into the vacuum source to give 3 L/min.
• Connect a low NO air source to the second nasal olive and adjust the gas flow
to ~3 L/min.
• With the subject comfortably seated, insert the nasal olives, making sure flow
to the vacuum source is maintained (olive not sealed against the skin).
• If the Accurate NO breath kit is used for expiratory resistance, connect the
Pressure line to one of the Luer port on the mouthpiece and use a Luer cap to
seal the other port. The oral exhalation flow rate is not important. The 50
mL/s restrictor is suitable for most subjects. ATS recommends a target pressure
of 10 cm H
2
O to ensure vellum closure.
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