GE Analytical Instruments ©2006 8-9 DLM 14291 Rev. A
After the subject has filled the replicate bags, remove the mouthpiece and filter.
The filter should be discarded, while the mouthpiece can be reused after
sterilization.
Collecting the Samples – Deadspace Discard Bag Kit
Sample collection using the Deadspace Discard Bag kit consists of five steps:
• Connecting a pre-cleaned bag to the filler.
• Instructing the subject on how to perform the maneuver.
• Exhaling to discard the gas in the physiological deadspace.
• Continued exhalation to collect the sample.
• Disconnecting the bag from the filler and sealing the bag.
Connecting the bag to the filler
To prepare the filler for sample collection:
• Stand the filler on the inspiratory filter.
• Push the female Luer adapter on the bag onto the male Luer fitting on the
filler and secure the bag by rotating the nut on the filler. The male Luer fitting
on the filler must contact and slightly depress the blue piece in the self-sealing
valve to allow gas to flow into the bag.
• Pull the valve stem on the filler to the out position (see Figure 8-4).
• Open a new viral and bacterial filter and connect the filter to the tapered end
on the top of the filler.
• Connect a sterile mouthpiece to the filter.
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