Prepare sample for application
using the pump
Follow the steps below to prepare for sample application using the pump.
ActionStep
Make sure that the sample inlet tubing that is to be used during the run is
placed in the sample container.
1
In the Process Picture pane, click on Inlet Valve and select Sample.
Result: The inlet valve opens the Sample inlet.
2
Connect a 25 to 30 mL syringe to the purge valve of one of the pump heads.3
Open the purge valve by turning it counter-clockwise one and a half turns.
Draw liquid slowly into the 25 to 30 mL syringe until the liquid reaches the
inlet valve.
4
Close the purge valve by turning it clockwise. Disconnect the syringe and
discard its contents.
5
ÄKTA go Operating Instructions 29360951 AC 83
6 Run a method
6.2 Prepare sample for loading