8 Troubleshooting
About this chapter
This chapter provides a list of the most commonly encountered problems that might
occur when operating ÄKTA go. For a more comprehensive list and a more detailed de-
scription of the actions to take, refer to the ÄKTA go User Manual.
Corrective actionPossible causeProblem
Replace the Flow restrictor back in
the flow path after the Conductivity
monitor.
The Flow restrictor has
been removed after the
UV cell.
Spike in the
UV signal
Prime the inlets and purge the pump,
see Section 5.2 Prime inlets and purge
pump heads, on page 64.
There is air in the system.
Prime the inlets and purge the pump,
see Section 5.2 Prime inlets and purge
pump heads, on page 64.
There is air in the pumpUnstable
conductivity
signal
Prime the inlets and purge the pump,
see Section 5.2 Prime inlets and purge
pump heads, on page 64.
There is air in the pump.Unstable
pressure
Lower the flow rate.The pressure is too high,
possibly due to running
the system at a flow rate
that is too high for the
column used.
Pressure
alarm issued
Replace the tubing. If this does not fix
the problem, refer to the ÄKTA go User
Manual to continue troubleshooting.
There is a kink in the tub-
ing, overtightened tubing
connections, or precipita-
tion in the flow path.
ÄKTA go Operating Instructions 29360951 AC 99
8 Troubleshooting