BiOptic Qsep100 - Page 116

BiOptic Qsep100

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116

Table of Contents

Welcome2
Copyright and Trademarks2
Symbols of Qsep Series2
Table of Contents3
Applications7
Packing List7
Cautions8
System Overview10
- Pre-Program Method and Cartridge11
  - Pre-Program Method11
- System Installation12
  - Software Requirements12
Q-Analyzer Installation Instruction13
- Software Installation13
- Software Mode18
  - USB Software Key20
  - Q-Editor License23
Start to Use Qsep™ Series26
- Operation of Qsep™ Series27
  - Connection Assistance28
  - Sequence and Method Setting32
- Cartridge Calibration40
- Edit Sample Position Settings42
- Recalibration44
- Capillary Clog Check45
- Purge Check Function48
- Home Function50
Q-Analyzer™ User Interface51
- Main Window51
  - System Overview52
  - System Status Column53
- Function Bar and Toolbar54
- Menu67
- Control Panel82
Result/Data Analysis88
- Result for Data Display88
- Comparison96
  - Export the Report of Compare Result103
- Total RNA Quality Analysis105
- Smear DNA Analysis (Gdna QC for NGS)107
- Peak Calling Function for Clinical Testing109
Appendix A-Troubleshooting112
Unpacking, Packing Process and Installation112
- Instrument112
- Q2：What Are the Minimum Computer Requirements and Recommendations112
- Q3：What Is the Size of the Result File113
- Q4：Do We Need to Install the Software First or Connect the Cable First113
- Q5：Can Power Adaptors be Used Alternately113
Instrument Not Working or Working Abnormally113
Before Use113
- Q1：Pressing the Machine Power, but Have no Response113
- Q2：How Should We Do When PC Cannot Detect the Connection of Qsep114
- Q3：How Should We Do When PC Cannot Connect with Qsep Wifi Mode114
- Q4：What Does the Light of Qair Mean115
During Use115
- Q5：If the Buffer Tray Can't Move Smoothly, How Should We Do115
- Q6：The Qair Works Frequently, over 3 Times/Min117
- Q7：Jet Error Window Pop-Out117
Cartridge Issues118
Before Use118
- Q1：HV Check Failed or Calibration Failed118
- Q2：What's Wrong with the Pop-Out Error Window119
During Use119
- Q3：How to Check if the Cartridge Is Clogged or Not and How to Rescue the Dry out Cartridge119
- Q4：What if the Cartridge Has Expired, but Still Has Runs Left120
- Q5：What if the Cartridge Has Sucked the Mineral Oil? How to Recover It120
- Q6：Anything Needs to Notice During Insert or Take out the Cartridge121
Storage122
- Q7：How to Store the Cartridge? and Relative Buffer122
Questions of Operation123
- Q1：How Do I Place the Qsep123
  - Alignment Marker and Size Marker123
- Q2：How Do I Prepare the Unknown Sample before Qsep Analysis123
- Q3：The Minimum Volume of Sample123
- Q4：The Sample Consumption (Per Run)124
- Q5：What Is the Air Pressure Range of each Instrument124
- Q6：What if We Use the Wrong Buffer124
- Q7：Will Dye or Organic Solvent Influence Cartridge? How to Pretreat the Sample125
- Q8：What Is the Difference between Basic Key and Advance Software Key125
- Q9：What Is the Difference between Q-Analyzer TM , Q-Editor TM and Q-Viewer TM126
- Q10：Why Should I Do Recalibration127
Unexpected Results128
- Q1：What Kind of Conditions will Cause Unstable Current128
- Q2：What to Do if Alignment Marker and Samples Are Migrating too Slowly and the Upper Alignment Marker Does Not Appear on the Result128
- Q3：Identify Wrong Alignment Marker or Size Marker129
- Q4：The Calculated Size Is Wrong131
- Q5：Is the Concentration Display in Result Table Reliable131
- Q6：The Current Is Stable, but There Is no Signal at All, Even the Alignment Marker Does Not Appear. RFU Near Zero131
- Q7：The Current Is Stable, but There Is no Signal Except the Alignment Marker132
- Q8：One of the 4 Channel Shows Different Result with Others132
Specific Application Issues (RNA, Protein133
- R1 RNA Cartridge133
- Q1：When Running RNA Samples, Are There any Special Things to Consider133
- Q2：What Is the Criteria of RQN133
- Q3：Why the Result Shows "N/A" in RQN134
- P2 Protein Cartridge135
- Q4：What Protein Alignment Marker Should I Use135
- Q5：Which Instrument Can I Use to Analyze Protein Sample? or What Labeling Dye Should I Choose136
- Q6：How to Do the Labeling Process136
Maintenance136
- Q1：How Often Should I Change the Marker and Buffer136
- Q2：What Happens if I Don't Change the Filter for a Long Time136
- Q3：How to Clean the Instrument Regularly136
Others137
- Q1：Should We Turn off the Instrument and Pump Every Day137
- Q2：How Should We Do before Discard the Cartridge137
- Report to Bioptic138

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