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cartridge that has been zeroed can be used to confirm that the machine is OK
or not.
• RFU near zero indicate there is no signal been detected. The major issue may
come from the light pathway (Ex/Em). Please refer to the service manual and
check if the J2 (LED) and J3 (PMT) connectors are loose. For further information,
please contact local distributor or BiOptic.
：
：
• The issue properly caused from the sample. You can run the size marker to
confirm).
Step1. You have to check the sample injection, the current is stable or not.
Step2. Check the sample concentration.
If the sample concentration is too low, you can select the “Low (<0.1
ng/µl)” in method. Besides, you can also use our N1 High sensitivity
cartridge to analyze these kind of samples.
Step3. Check the salt concentration of the samples.
If the salt concentration is too high, please follow the 4-Q2 to dilute the
sample with 0.1X dilution buffer or Clean up the sample will be better.
：
：
Step1. Check the samples which are belong to that failed channel. Make sure all
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