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1. The result needs to have two peaks (more than two or less than two will
also failed) and the S/N must be 60 times of noise.
2. Peak time must be within the specified range, cannot be shift too far.
• If the calibration failed, user can do it again, and it will extend 10s of the HV
purge (10n, n= calibration number) and 30s of separation in next
calibration.
• The cartridge will be locked after 10 times failed.
*Note: make sure user place above 20µl of 20bp& 1kb in MA1 (M) position properly.
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• Using the purge station to see whether the gel drop comes out. If not, soak
the cartridge tip in the beaker with hot water for 10-15mins and then purge
again.
• It will be even better to soak and purge at the same time
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