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Qsep100
BiOptic Qsep100 User Manual
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124
：
：
•
For diff
erent c
artridges, all the sample co
n
sumption is < 0.1 µl per run.
Q5
：
What is the air pre
ssure range of each instrument?
：
Please ref
er to Oper
ation manual- Hardw
are.
Qsep
100
Qsep
1
Qsep
400
*Qsep
100
,
which was pr
oduced bef
ore 2016 Sep., d
oe
s not hav
e this function
because it uses the old motherboar
d.
：
：
•
Using dilution buff
er to do the separ
ation, the pea
ks pat
ter
n
becomes weir
d
and the current r
aise.
123
125
Table of Contents
Welcome
2
Copyright and Trademarks
2
Symbols of Qsep Series
2
Table of Contents
3
Applications
7
Packing List
7
Cautions
8
System Overview
10
Pre-Program Method and Cartridge
11
Pre-Program Method
11
System Installation
12
Software Requirements
12
Q-Analyzer Installation Instruction
13
Software Installation
13
Software Mode
18
USB Software Key
20
Q-Editor License
23
Start to Use Qsep™ Series
26
Operation of Qsep™ Series
27
Connection Assistance
28
Sequence and Method Setting
32
Cartridge Calibration
40
Edit Sample Position Settings
42
Recalibration
44
Capillary Clog Check
45
Purge Check Function
48
Home Function
50
Q-Analyzer™ User Interface
51
Main Window
51
System Overview
52
System Status Column
53
Function Bar and Toolbar
54
Instrument
54
Results
59
Comparison
65
Real Time
66
Menu
67
File
67
Edit
70
Tool
74
View
77
Window
78
Setting
78
Language
79
Help
80
Control Panel
82
Main
82
Method
84
Direct Control (Partial Advanced Only)
86
Board Setting (Advanced Only)
87
Result/Data Analysis
88
Result for Data Display
88
Calculation
91
Reference Marker Table
93
Improve Calculate Accuracy
95
Comparison
96
Export the Report of Compare Result
103
Total RNA Quality Analysis
105
Smear DNA Analysis (Gdna QC for NGS)
107
Peak Calling Function for Clinical Testing
109
Appendix A-Troubleshooting
112
Unpacking, Packing Process and Installation
112
Instrument
112
Q2：What Are the Minimum Computer Requirements and Recommendations
112
Q3：What Is the Size of the Result File
113
Q4：Do We Need to Install the Software First or Connect the Cable First
113
Q5：Can Power Adaptors be Used Alternately
113
Instrument Not Working or Working Abnormally
113
Before Use
113
Q1：Pressing the Machine Power, but Have no Response
113
Q2：How Should We Do When PC Cannot Detect the Connection of Qsep
114
Q3：How Should We Do When PC Cannot Connect with Qsep Wifi Mode
114
Q4：What Does the Light of Qair Mean
115
During Use
115
Q5：If the Buffer Tray Can't Move Smoothly, How Should We Do
115
Q6：The Qair Works Frequently, over 3 Times/Min
117
Q7：Jet Error Window Pop-Out
117
Cartridge Issues
118
Before Use
118
Q1：HV Check Failed or Calibration Failed
118
Q2：What's Wrong with the Pop-Out Error Window
119
During Use
119
Q3：How to Check if the Cartridge Is Clogged or Not and How to Rescue the Dry out Cartridge
119
Q4：What if the Cartridge Has Expired, but Still Has Runs Left
120
Q5：What if the Cartridge Has Sucked the Mineral Oil? How to Recover It
120
Q6：Anything Needs to Notice During Insert or Take out the Cartridge
121
Storage
122
Q7：How to Store the Cartridge? and Relative Buffer
122
Questions of Operation
123
Q1：How Do I Place the Qsep
123
Alignment Marker and Size Marker
123
Q2：How Do I Prepare the Unknown Sample before Qsep Analysis
123
Q3：The Minimum Volume of Sample
123
Q4：The Sample Consumption (Per Run)
124
Q5：What Is the Air Pressure Range of each Instrument
124
Q6：What if We Use the Wrong Buffer
124
Q7：Will Dye or Organic Solvent Influence Cartridge? How to Pretreat the Sample
125
Q8：What Is the Difference between Basic Key and Advance Software Key
125
Q9：What Is the Difference between Q-Analyzer TM , Q-Editor TM and Q-Viewer TM
126
Q10：Why Should I Do Recalibration
127
Unexpected Results
128
Q1：What Kind of Conditions will Cause Unstable Current
128
Q2：What to Do if Alignment Marker and Samples Are Migrating too Slowly and the Upper Alignment Marker Does Not Appear on the Result
128
Q3：Identify Wrong Alignment Marker or Size Marker
129
Q4：The Calculated Size Is Wrong
131
Q5：Is the Concentration Display in Result Table Reliable
131
Q6：The Current Is Stable, but There Is no Signal at All, Even the Alignment Marker Does Not Appear. RFU Near Zero
131
Q7：The Current Is Stable, but There Is no Signal Except the Alignment Marker
132
Q8：One of the 4 Channel Shows Different Result with Others
132
Specific Application Issues (RNA, Protein
133
R1 RNA Cartridge
133
Q1：When Running RNA Samples, Are There any Special Things to Consider
133
Q2：What Is the Criteria of RQN
133
Q3：Why the Result Shows "N/A" in RQN
134
P2 Protein Cartridge
135
Q4：What Protein Alignment Marker Should I Use
135
Q5：Which Instrument Can I Use to Analyze Protein Sample? or What Labeling Dye Should I Choose
136
Q6：How to Do the Labeling Process
136
Maintenance
136
Q1：How Often Should I Change the Marker and Buffer
136
Q2：What Happens if I Don't Change the Filter for a Long Time
136
Q3：How to Clean the Instrument Regularly
136
Others
137
Q1：Should We Turn off the Instrument and Pump Every Day
137
Q2：How Should We Do before Discard the Cartridge
137
Report to Bioptic
138
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BiOptic Qsep100 Specifications
General
Brand
BiOptic
Model
Qsep100
Category
Medical Equipment
Language
English
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BiOptic Qsep Series
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