34 www.millipore.com
3. Add 0.1 mL of water to a 1 mL pipette. Insert bottom
of pipette into the end of the permeate tubing (silicone,
translucent).
4. Measure airflow by measuring the displacement of water in
the pipette for 30 seconds. Multiply the displaced volume by 2
to calculate the airflow (mL/min) and record.
5. Remove the pipette from the permeate tubing (silicone,
translucent). Open the retentate valve slowly to release the air
pressure in the system.
6. Reconnect the pump inlet tubing (silicone, translucent) to the
tank outlet valve.
The Pellicon XL device is integral if the measured airflow is
lessthan or equal to the airflow specification on the Certificate of
Quality.
Preconditioning
1. Place end of permeate tubing silicone (translucent) in waste
collection vessel.
2. Remove reservoir cover and fill reservoir with 50 mL of an
appropriate buffer. Remove plug from VENT port.
3. Open tank outlet valve. Turn pump on - increase pump speed
until feed pressure gauge reads 1.38 Bar (20 psi).
Note: Check system connections for leaks—tighten connec-
tions if required
4. Continue pumping to the waste collection vessel until the level
in the reservoir drops to the bottom of the reservoir stir bar
well.(Stop pump before air is pumped into the system.). Turn
pump off.
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