5. Use directional keys to correct the volume on the flashing display
6. Press the Enter key to save the calibration setting. Once calibrated, a lower case "c" appears
next to the tubing (e.g. 73c)
7. To confirm tubing calibration, press CHECK to dispense calibration volume using calibrated
tubing and weigh dispensed volume
NOTE: If the adjusted calibration is high, "Err" will appear in the display. If this occurs, press the CAL
control and repeat the calibration procedure. The microprocessor will retain one special
calibration value per tubing size, even when power is turned off. The next calibration will
replace the existing value.
System Functionality
8.1 Pump Controls
The Pump Control Interface can be used to interact with all the features of the system. This screen
will appear when the KF Comm software is opened in Excel. When needed, the functions and
setpoints are accessible through the touchpad on the main pump display. See the Pump Control
layout in section 7.9.1 KF COMM Pump Control User Interface for further information using the Pump
Control Interface on the PC.
8.1.1 Overview
The TFF system alternates through different functions based on user-defined set-points suitable for
the user’s application—these combinations of functions are Pump Modes. All Pump Modes are
composed of different sequences of Concentration and Diafiltration functions. Refer to Section 12
Basic Concepts of Tangential Flow Filtration.
a. Concentration: Reduce volume of process solution
b. Diafiltration: Wash permeable molecules out of the process solution
Auxiliary Components are associated with the following most commonly used automated functions
and actions:
c. Feed Scale: Weighs feed reservoir to monitor Concentration and Diafiltration functions
d. Permeate Scale: Weighs permeate reservoir to monitor Concentration and Diafiltration
functions
e. Diafiltration Pump: Replenishes loss feed reservoir volume during diafiltration function;
usually Auxiliary Pump 1 in most modes
f. Permeate Pump: Controls permeation rate for MF applications; usually Auxiliary Pump 2 in
most modes
g. Valve 1: Control TMP, Retentate, Inlet or Permeate Pressure
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