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REPLIGEN Spectrum KR2i User Manual

REPLIGEN Spectrum KR2i
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48 400-12355-000 Rev. 03
KR2i / KMPi TFF Systems
System Functionality
NOTE: In CFC and CF/D/C mode, there is a Constant Feed Concentration step (the name "CFC" is
shortened to "CF" due to character constraints for "CF/D/C" mode). The CFC function is a
modified Concentration function. During the CFC step of the two Pump Modes, Auxiliary Pump
1 is used as a Constant Feed Pump that pumps Extra Feed Volume from the Extra Feed
Reservoir to the Feed Reservoir. The flow rate of the Constant Feed Pump adjusts to maintain
the starting weight on the Feed Scale-- it will continue doing this until the end of the Constant
Feed Concentration step. The Constant Feed Concentration step ends when the desired
Concentration Factor set-point has been achieved. The CFC function is useful for when a
vacuum can not be created between the Extra Feed Reservoir and Feed Reservoir.
8.3 Running the TFF System
Prior to running application, set Hardware (see Section 7.9.2 #5 for Hardware Setup details)
and Auxiliary Pump configurations through either the TFF system (see Section 7.9.2 for
Auxiliary Pump Settings) or KF Comm Pump Control (see Section 7.10.4 for Pump Control)
1. Set the flow rate of the TFF system in the Main Screen
2. Select a Pump Mode suitable for application (see Section 12.4 for Sample Applications)
3. Input set-points required for the Pump Mode
4. Press the Start/Pause soft button on the TFF system to start Pump Mode or the green
"START" button on top line of Pump Control window. When each set-point is reached during
functions, Pump Mode will seamlessly continue to next function until the Pump Mode’s set
of functions are completed. Pump Mode will then end automatically.
a. M Mode: Manual Mode
b. C Mode: Concentration Mode
c. C/D Mode: Concentration/Diafiltration Mode
d. C/D/C Mode: Concentration 1/Diafiltration/Concentration 2 Mode
e. C/D/D/C Mode: Concentration 1/Diafiltration 1/Diafiltration 2/ Concentration 2
Mode
f. CFC Mode: Constant Feed Concentration Mode
g. CF/D/C Mode: Constant Feed/Diafiltration/Concentration Mode
h.
(KF Comm Only) Ctrl Mode: Control Mode
8.4 Pump Mode Status Timeout Display
Use the Pump Mode Status Timeout display to monitor Pump Mode progress. Depending on the
Pump Mode, the Timeout display will either show scale weight values, Concentration
Factor/Diafiltration Volumes (CF/DV), or KONDUiT readings when attached. Manual Mode will
always show scale weight values.

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REPLIGEN Spectrum KR2i Specifications

General IconGeneral
BrandREPLIGEN
ModelSpectrum KR2i
CategoryLaboratory Equipment
LanguageEnglish

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