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BioMate 3 series
Thermo BioMate 3 series User Manual
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BioMate™ 3 Series
S
pectrophotometers
Operator
’
s Manual
2
Table of Contents
Default Chapter
13
Table of Contents
13
CHAPTER 1 - Setting up the Instrument
17
Setting up the Instrument
17
Setting Utility Parameters
17
Selecting the Language
17
Setting the Date and Time
18
Selecting Standby Settings
18
Setting Baseline Expiration Time
18
Setting the Screen Contrast
18
Loading Paper in the Internal Printer
18
Setting the Utility Parameters for the Printer
19
Selecting and Positioning Glassware
19
Z-Dimensions
20
CHAPTER 2 - Using "Biotests" Software
21
Overview
21
Table of Parameters
21
Entering Information & Commands
21
Types of Parameter Entries
21
Keypad Layout
21
Smartstart Feature
22
Setting up a Single-Test Smartstart
22
Setting up a Multiple-Test Smartstart
22
Nucleic Acid Measurements
22
DNA (260/280 and DNA 260/230)
23
DNA with Scan (260/280) and DNA with Scan (260/230)
24
Dsdna, Ssdna, RNA and Oligos (Entered Factor) Direct or UV Measurements
25
Protein Measurements
28
Bradford (Standard & Micro), Lowry (Standard & Micro), BCA (Standard & Micro) and Biuret Measurements
28
Direct UV (280) and Direct UV (205)
29
Warburg-Christian
30
Cell Growth
31
Setting Test Parameters
31
Measuring the Sample
31
Oligo Calculator
32
Using the Oligo Calculator
32
CHAPTER 3 - Using "General Tests" Software
33
General Information
33
Editing and Loading Saved Tests
33
Specifying Names for Tests
33
Specifying Concentration Units
33
Using the Smartstart Feature
34
Running the Cell Correction Program
35
Taking Measurements
37
Saving Tests
37
Basic Absorbance/%T Measurements
38
Setting the Wavelength
38
Measuring a Blank
38
Measuring Unknowns
38
Basic Concentration Measurements
38
Setting the Wavelength & Mode
39
Measuring a Blank
39
Measuring a Standard
39
Entering a Factor
40
Measuring Unknowns
40
Advanced A/%T/C - Absorbance & %Transmittance Measurements
40
Recalling a Test
40
Setting up Test Parameters
41
Taking Measurements
41
Advanced A/%T/C - Concentration Measurements
41
Recalling a Test
42
Setting up Test Parameters
42
Measuring a Standard
42
Entering a Factor
43
Measuring Unknowns
43
Standard Curve
43
Recalling a Standard Curve
44
Setting the Parameters for a Standard Curve
44
Measuring the Standards for a Standard Curve
44
Measuring Unknowns
45
Editing a Standard Curve
45
Absorbance Ratio
46
Recalling a Test
47
Setting up Test Parameters
47
Measuring Unknowns
47
Absorbance Difference
48
Recalling a Test
48
Setting up Test Parameters
48
Measuring Unknowns
48
Kinetics
49
Recalling a Test
49
Setting up Test Parameters
49
Measuring Unknowns
49
Rescaling & Recalculating Kinetics Results
50
Rescaling & Recalculating Tabular Kinetics Results
51
Survey Scan/Scanning
52
Recalling a Test
52
Setting up Test Parameters
52
Collecting a Baseline Scan
52
Scanning an Unknown
53
Viewing & Manipulating Scan Data
53
3-Point Net
56
Taking Measurements
56
Recalling a Test
56
Setting up Test Parameters
56
Taking Measurements
58
CHAPTER 4 - Using the Performance Validation Program
61
Overview
61
Accessing the Performance Validation Tests
61
Troubleshooting Checklist
61
Wavelength Accuracy - Internal
61
Wavelength Accuracy - Standards
62
Adding Wavelengths
62
Deleting Wavelengths
63
Photometric Accuracy
63
Selecting the Mode
63
Adding Standards
63
Running the Test
63
Noise Measurement
64
Running the Test
64
Internal Printer Test
65
RS232C Test
66
CHAPTER 5 - Connecting & Using Accessories
67
General Information
67
Cell Holders & Cell Holder Accessories
67
Changing Cell Holders
68
Internal Printer
69
Installing the Internal Printer
69
Loading Paper in the Internal Printer
69
External Computers
69
CHAPTER 6 - Performing Maintenance Procedures
71
Routine Care
71
Cleaning
71
Cleaning and Maintenance of Cells
71
Cleaning the Windows of the Sample Compartment
72
Changing the Fuse
72
Replacement Parts
73
4
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Thermo BioMate 3 series Specifications
General
Brand
Thermo
Model
BioMate 3 series
Category
Measuring Instruments
Language
English
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