30
5
Diopter Adjustment
Diopter adjustment compensates for differences in eyesight between your left and right eyes. After
the correct adjustment, you will find the observation with both eyes easier and the focus shift is
reduced when switched to different objectives. Be sure to adjust the diopter adjustment rings on
both eyepieces.
1 Turn the diopter adjustment rings on both eyepieces to align their engraved lines with the
edge of the outer tube of the eyepiece. (This is the standard position for diopter adjustment.)
2 Focus on the sample with the 10x objective following the steps of bright-field microscopy
(p.16, 17).
3 Bring the 50x objective into the optical path and focus on the sample by turning the coarse/
fine focus knobs.
4 Bring the 5x or 10x objective into the optical path.
5 Focus on the sample by turning the diopter adjustment ring on the right eyepiece (not the
coarse/fine focus knobs).
Look through the left eyepiece with your left eye, and the right eyepiece with your right eye,
to focus on the sample with the diopter adjustment rings.
6 Repeat steps 3 to 5 with the 50x and 5x (or 10x) objectives until the image stays in focus
even though the objective magnification is changed.
F
.
STOP
A
.
STOP
BF
DF
1
0
0
0
0
1
0
0
IN
O
U
T
Engraved
line
Diopter adjustment standard position
Diopter
adjustment
ring
Outer tube
edge
Positioning
grooves
6
Interpupillary Distance Adjustment
Before adjusting the interpupillary distance,
perform the steps of bright-field microscopy (p.16,
17) and focus on the image with the 10x objective.
Adjust the interpupillary distance so that the
viewfields for both eyes are at the same position on
the sample.
Doing so will make observation through the
binocular eyepieces with both eyes easier.
The scale on the binocular part is useful in order to
memorize your interpupillary distance for the next
time.
F
.
STOP A
.
STOP
BF
DF
10
0
0
0
100
IN
O
U
T
Merge the view fields into one.
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